Three-dimensional culture of epidermal cells on ordered cellulose scaffolds

Tomoko Seyama, Eun Young Suh, Tetsuo Kondo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An ordered cellulose film scaffold, termed a nematic ordered cellulose (NOC) template, had unique surface properties and successfully induced the establishment of a three-dimensional (3D), hierarchical structure of epidermal cells by cell attachment and subsequent culture. Initially, the scaffold surface properties were characterized through contact angle measurements and atomic force microscopy to evaluate appropriate hydrophobicity and orientation of molecular chains for 3D culture. The template surfaces exhibited higher hydrophobicity, in the range of 70-75°, than usual cellulose films and appeared suitable for surface cell adhesion. In fact, epidermal cells successfully attached and proliferated favorably on the NOC templates, similar to development in normal culture flasks. Furthermore, the NOC film, as a semipermeable template, was also employed to allow 3D proliferation of epidermal cell layers in the perpendicular direction. The template proved to be suitable as a 3D cell culture device, resulting in the proposal that the construction processes of these 3D cell layers followed the basic concept of skin formation.

Original languageEnglish
Article number025010
JournalBiofabrication
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1 2013

Fingerprint

Cellulose films
Scaffolds
Cellulose
Cell Culture Techniques
Hydrophobicity
Surface properties
Cells
Surface Properties
Hydrophobic and Hydrophilic Interactions
Cell adhesion
Angle measurement
Cell culture
Contact angle
Concept Formation
Atomic force microscopy
Skin
Atomic Force Microscopy
Cell Adhesion
Cell Proliferation
Equipment and Supplies

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Three-dimensional culture of epidermal cells on ordered cellulose scaffolds. / Seyama, Tomoko; Suh, Eun Young; Kondo, Tetsuo.

In: Biofabrication, Vol. 5, No. 2, 025010, 01.06.2013.

Research output: Contribution to journalArticle

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