Three-dimensional electron microscopy of the photosystem II core complex

Kouta Mayanagi, Takashi Ishikawa, Chikashi Toyoshima, Yorinao Inoue, Katsuyoshi Nakazato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A three-dimensional image of the spinach photosystem II core complex composed of CP47, D1, D2, cytochrome b-559, and psbI gene product was reconstructed at 20-Å resolution from the two-dimensional crystals negatively stained with phosphotungstate. Confirming the previous proposal, the crystal had a p22121 symmetry. One PSH core complex was measured to be 80 x 80 Å in the membrane plane and 88 Å normal to it. The mass distribution was asymmetric about the lipid bilayer, consistent with predictions from the amino acid sequences. The lumenal mass consisted of three domains forming a characteristic triangular platform with another domain on top of it. Three stromal domains were smaller and linearly arranged. Due to strong stain exclusion in the hydrophobic core part of the lipid bilayer, the transmembrane region appeared to be imaged with a reversed contrast. Inverting the contrast resulted in a reasonable density distribution for that part. Thus, though the information on the transmembrane region is limited, the domain structure of the PSII core complex was revealed and allowed us to propose a model for the arrangement of subunits in the PSII core complex.

Original languageEnglish
Pages (from-to)211-224
Number of pages14
JournalJournal of Structural Biology
Volume123
Issue number3
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology

Fingerprint Dive into the research topics of 'Three-dimensional electron microscopy of the photosystem II core complex'. Together they form a unique fingerprint.

  • Cite this