Three-dimensional electron microscopy of the reverse gyrase from Sulfolobus tokodaii

Kyoko Matoba, Kouta Mayanagi, Syo Nakasu, Akihiko Kikuchi, Kosuke Morikawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Reverse gyrase is a type IA topoisomerase, found in various hyperthermophiles and promotes ATP-dependent positive super-coiling of DNA. Electron microscopy combined with single particle analyses revealed the three-dimensional structure of the DNA-free Sulfolobus tokodaii reverse gyrase and two-dimensional average images of both the protein alone and that complexed with double-stranded DNA. The 23 Å resolution map exhibited a parallelogrammatic morphology of 110 × 87 × 43 Å, which is in good agreement with the crystal structure of the Archaeoglobus fulgidus reverse gyrase. The average image of the complex revealed that the monomeric enzyme binds DNA duplex. Together with this average image of the complex, the three-dimensional map implies that, at the beginning of the supercoiling reaction, DNA is bound within a 10-20 Å wide cleft in the helicase-like domain. We also speculate that DNA may pass through a 20 Å wide hole at the end of the cleft.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number4
DOIs
Publication statusPublished - Nov 7 2002

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Sulfolobus
Electron microscopy
Electron Microscopy
DNA
Archaeoglobus fulgidus
Three-Dimensional Imaging
DNA reverse gyrase
Adenosine Triphosphate
Crystal structure
Enzymes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Three-dimensional electron microscopy of the reverse gyrase from Sulfolobus tokodaii. / Matoba, Kyoko; Mayanagi, Kouta; Nakasu, Syo; Kikuchi, Akihiko; Morikawa, Kosuke.

In: Biochemical and Biophysical Research Communications, Vol. 297, No. 4, 07.11.2002, p. 749-755.

Research output: Contribution to journalArticle

Matoba, Kyoko ; Mayanagi, Kouta ; Nakasu, Syo ; Kikuchi, Akihiko ; Morikawa, Kosuke. / Three-dimensional electron microscopy of the reverse gyrase from Sulfolobus tokodaii. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 297, No. 4. pp. 749-755.
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