Three-dimensional model of intracellular and intercellular Ca2+ waves propagation in endothelial cells

Toshihiro Sera, Shingo Komine, Masataka Arai, Yasuhiro Sunaga, Hideo Yokota, Susumu Kudo

Research output: Contribution to journalArticle

Abstract

Intracellular and intercellular Ca2+ waves play key roles in cellular functions, and focal stimulation triggers Ca2+ wave propagation from stimulation points to neighboring cells, involving localized metabolism reactions and specific diffusion processes. Among these, inositol 1,4,5-trisphosphate (IP3) is produced at membranes and diffuses into the cytoplasm to release Ca2+ from endoplasmic reticulum (ER). In this study, we developed a three-dimensional (3D) simulation model for intercellular and intracellular Ca2+ waves in endothelial cells (ECs). 3D model of 2 cells was reconstructed from confocal microscopic images and was connected via gap junctions. Cells have membrane and cytoplasm domains, and metabolic reactions were divided into each domain. Finally, the intracellular and intercellular Ca2+ wave propagations were induced using microscopic stimulation and were compared between numerical simulations and experiments. The experiments showed that initial sharp increases in intracellular Ca2+ occurred approximately 0.3 s after application of stimuli. In addition, Ca2+ wave speeds remained constant in cells, with intracellular and intercellular speeds of approximately 35 and 15 μm/s, respectively. Simulations indicated initial increases in Ca2+ concentrations at points of stimulation, and these were then propagated across stimulated and neighboring cells. In particular, initial rapid increases in intracellular Ca2+ were delayed and subsequent intracellular and intercellular Ca2+ wave speeds were approximately 25 and 12 μm/s, respectively. Simulation results were in agreement with those from cell culture experiments, indicating the utility of our 3D model for investigations of intracellular and intercellular messaging in ECs.

Original languageEnglish
Pages (from-to)781-786
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume505
Issue number3
DOIs
Publication statusPublished - Nov 2 2018

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Endothelial cells
Wave propagation
Endothelial Cells
Cytoplasm
Inositol 1,4,5-Trisphosphate
Gap Junctions
Experiments
Cell membranes
Cell culture
Metabolism
Endoplasmic Reticulum
Cell Culture Techniques
Cell Membrane
Membranes
Computer simulation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Three-dimensional model of intracellular and intercellular Ca2+ waves propagation in endothelial cells. / Sera, Toshihiro; Komine, Shingo; Arai, Masataka; Sunaga, Yasuhiro; Yokota, Hideo; Kudo, Susumu.

In: Biochemical and Biophysical Research Communications, Vol. 505, No. 3, 02.11.2018, p. 781-786.

Research output: Contribution to journalArticle

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