Three-dimensional phase-contrast imaging of single floating cells

Hiroaki Kobayashi, Ichirou Ishimaru, Toshiki Yasokawa, Katsumi Ishizaki, Shigeki Kuriyama, Tsutomu Masaki, Seiji Nakai, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A three-dimensional phase-contrast imaging technique that does not involve fluorescent labeling has been developed for observing floating cells. In this method, a single floating cell is made to rotate and images are acquired at several orientations of the cell using a phase-contrast microscope. From these two-dimensional phase-contrast images, three-dimensional cross-sectional images are obtained using the conventional computed tomography algorithm. This proposed method enabled successful rotation of a floating cell (a breast cancer cell line) and reconstruction of three-dimensional phase-contrast images. In these reconstructed three-dimensional images, the distribution of cell organelles is obtained and the cell nucleus is clearly distinguishable.

Original languageEnglish
Article number241117
JournalApplied Physics Letters
Volume89
Issue number24
DOIs
Publication statusPublished - Dec 29 2006

Fingerprint

phase contrast
floating
cells
organelles
cultured cells
imaging techniques
breast
marking
tomography
cancer
microscopes
nuclei

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Kobayashi, H., Ishimaru, I., Yasokawa, T., Ishizaki, K., Kuriyama, S., Masaki, T., ... Tanaka, N. (2006). Three-dimensional phase-contrast imaging of single floating cells. Applied Physics Letters, 89(24), [241117]. https://doi.org/10.1063/1.2402229

Three-dimensional phase-contrast imaging of single floating cells. / Kobayashi, Hiroaki; Ishimaru, Ichirou; Yasokawa, Toshiki; Ishizaki, Katsumi; Kuriyama, Shigeki; Masaki, Tsutomu; Nakai, Seiji; Takegawa, Kaoru; Tanaka, Naotaka.

In: Applied Physics Letters, Vol. 89, No. 24, 241117, 29.12.2006.

Research output: Contribution to journalArticle

Kobayashi, H, Ishimaru, I, Yasokawa, T, Ishizaki, K, Kuriyama, S, Masaki, T, Nakai, S, Takegawa, K & Tanaka, N 2006, 'Three-dimensional phase-contrast imaging of single floating cells', Applied Physics Letters, vol. 89, no. 24, 241117. https://doi.org/10.1063/1.2402229
Kobayashi H, Ishimaru I, Yasokawa T, Ishizaki K, Kuriyama S, Masaki T et al. Three-dimensional phase-contrast imaging of single floating cells. Applied Physics Letters. 2006 Dec 29;89(24). 241117. https://doi.org/10.1063/1.2402229
Kobayashi, Hiroaki ; Ishimaru, Ichirou ; Yasokawa, Toshiki ; Ishizaki, Katsumi ; Kuriyama, Shigeki ; Masaki, Tsutomu ; Nakai, Seiji ; Takegawa, Kaoru ; Tanaka, Naotaka. / Three-dimensional phase-contrast imaging of single floating cells. In: Applied Physics Letters. 2006 ; Vol. 89, No. 24.
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