Three-dimensional phase-contrast imaging of single floating cells

Hiroaki Kobayashi, Ichirou Ishimaru, Toshiki Yasokawa, Katsumi Ishizaki, Shigeki Kuriyama, Tsutomu Masaki, Seiji Nakai, Kaoru Takegawa, Naotaka Tanaka

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4 Citations (Scopus)

Abstract

A three-dimensional phase-contrast imaging technique that does not involve fluorescent labeling has been developed for observing floating cells. In this method, a single floating cell is made to rotate and images are acquired at several orientations of the cell using a phase-contrast microscope. From these two-dimensional phase-contrast images, three-dimensional cross-sectional images are obtained using the conventional computed tomography algorithm. This proposed method enabled successful rotation of a floating cell (a breast cancer cell line) and reconstruction of three-dimensional phase-contrast images. In these reconstructed three-dimensional images, the distribution of cell organelles is obtained and the cell nucleus is clearly distinguishable.

Original languageEnglish
Article number241117
JournalApplied Physics Letters
Volume89
Issue number24
DOIs
Publication statusPublished - Dec 29 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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  • Cite this

    Kobayashi, H., Ishimaru, I., Yasokawa, T., Ishizaki, K., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., & Tanaka, N. (2006). Three-dimensional phase-contrast imaging of single floating cells. Applied Physics Letters, 89(24), [241117]. https://doi.org/10.1063/1.2402229