TY - JOUR
T1 - Three-dimensional reconstruction of intramuscular collagen networks of bovine muscle
T2 - A demonstration by an immunohistochemical/confocal laser-scanning microscopic method: RAPID COMMUNICATION
AU - Nakamura, Yoshi Nori
AU - Iwamoto, Hisao
AU - Yamaguchi, Takahiro
AU - Ono, Yoshitaka
AU - Nakanishi, Yoshitaka
AU - Tabata, Shoji
AU - Nishimura, Shotaro
AU - Gotoh, Takafumi
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2007/8
Y1 - 2007/8
N2 - ABSTRACT We undertook a three-dimensional reconstruction of intramuscular collagen networks of bovine muscle using an immunohistochemical/confocal laser-scanning microscopic method. By immunohistochemical staining, type I and III collagens were observed mainly in the perimysium, while type IV collagen was observed in the endomysium. On the other hand, type V and VI collagens were observed in both the perimysium and endomysium. By confocal laser-scanning microscopy, the collagen observed in the perimysium was three-dimensionally reconstructed as plate-shaped layers whereas the collagen observed in the endomysium surrounded myofibers. The three-dimensionally reconstructed observations using immunohistochemical/confocal laser-scanning microscopic method is useful for investigating collagen networks in muscle.
AB - ABSTRACT We undertook a three-dimensional reconstruction of intramuscular collagen networks of bovine muscle using an immunohistochemical/confocal laser-scanning microscopic method. By immunohistochemical staining, type I and III collagens were observed mainly in the perimysium, while type IV collagen was observed in the endomysium. On the other hand, type V and VI collagens were observed in both the perimysium and endomysium. By confocal laser-scanning microscopy, the collagen observed in the perimysium was three-dimensionally reconstructed as plate-shaped layers whereas the collagen observed in the endomysium surrounded myofibers. The three-dimensionally reconstructed observations using immunohistochemical/confocal laser-scanning microscopic method is useful for investigating collagen networks in muscle.
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U2 - 10.1111/j.1740-0929.2007.00460.x
DO - 10.1111/j.1740-0929.2007.00460.x
M3 - Article
AN - SCOPUS:34547321013
VL - 78
SP - 445
EP - 447
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 4
ER -