Three-dimensional reconstruction of intramuscular collagen networks of bovine muscle: A demonstration by an immunohistochemical/confocal laser-scanning microscopic method: RAPID COMMUNICATION

Yoshi Nori Nakamura, Hisao Iwamoto, Takahiro Yamaguchi, Yoshitaka Ono, Yoshitaka Nakanishi, Shoji Tabata, Shotaro Nishimura, Takafumi Gotoh

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4 Citations (Scopus)

Abstract

ABSTRACT We undertook a three-dimensional reconstruction of intramuscular collagen networks of bovine muscle using an immunohistochemical/confocal laser-scanning microscopic method. By immunohistochemical staining, type I and III collagens were observed mainly in the perimysium, while type IV collagen was observed in the endomysium. On the other hand, type V and VI collagens were observed in both the perimysium and endomysium. By confocal laser-scanning microscopy, the collagen observed in the perimysium was three-dimensionally reconstructed as plate-shaped layers whereas the collagen observed in the endomysium surrounded myofibers. The three-dimensionally reconstructed observations using immunohistochemical/confocal laser-scanning microscopic method is useful for investigating collagen networks in muscle.

Original languageEnglish
Pages (from-to)445-447
Number of pages3
JournalAnimal Science Journal
Volume78
Issue number4
DOIs
Publication statusPublished - Aug 2007

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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