Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli

Yuriko Yamagata, Masato Kato, Kyoko Odawara, Yoshiteru Tokuno, Yoko Nakashima, Nobuko Matsushima, Kohei Yasumura, Ken Ichi Tomita, Kenji Ihara, Yoshimitsu Fujii, Yusaku Nakabeppu, Mutsuo Sekiguchi, Satoshi Fujii

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

The three-dimensional structure of Escherichia coli 3-methyladenine DNA glycosylase II, which removes numerous alkylated bases from DNA, was solved at 2.3 Å resolution. The enzyme consists of three domains: one α + β fold domain with a similarity to one-half of the eukaryotic TATA box-binding protein, and two all α-helical domains similar to those of Escherichia coli endonuclease III with combined N-glycosylase/abasic lyase activity. Mutagenesis and model-building studies suggest that the active site is located in a cleft between the two helical domains and that the enzyme flips the target base out of the DNA duplex into the active-site cleft. The structure of the active site implies broad substrate specificity and simple N-glycosylase activity.

Original languageEnglish
Pages (from-to)311-319
Number of pages9
JournalCell
Volume86
Issue number2
DOIs
Publication statusPublished - Jul 26 1996

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DNA Repair Enzymes
Escherichia coli
Catalytic Domain
TATA-Box Binding Protein
Mutagenesis
Lyases
Endonucleases
DNA
Enzymes
Substrate Specificity
Substrates
DNA-3-methyladenine glycosidase II

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Yamagata, Y., Kato, M., Odawara, K., Tokuno, Y., Nakashima, Y., Matsushima, N., ... Fujii, S. (1996). Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli. Cell, 86(2), 311-319. https://doi.org/10.1016/S0092-8674(00)80102-6

Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli. / Yamagata, Yuriko; Kato, Masato; Odawara, Kyoko; Tokuno, Yoshiteru; Nakashima, Yoko; Matsushima, Nobuko; Yasumura, Kohei; Tomita, Ken Ichi; Ihara, Kenji; Fujii, Yoshimitsu; Nakabeppu, Yusaku; Sekiguchi, Mutsuo; Fujii, Satoshi.

In: Cell, Vol. 86, No. 2, 26.07.1996, p. 311-319.

Research output: Contribution to journalArticle

Yamagata, Y, Kato, M, Odawara, K, Tokuno, Y, Nakashima, Y, Matsushima, N, Yasumura, K, Tomita, KI, Ihara, K, Fujii, Y, Nakabeppu, Y, Sekiguchi, M & Fujii, S 1996, 'Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli', Cell, vol. 86, no. 2, pp. 311-319. https://doi.org/10.1016/S0092-8674(00)80102-6
Yamagata, Yuriko ; Kato, Masato ; Odawara, Kyoko ; Tokuno, Yoshiteru ; Nakashima, Yoko ; Matsushima, Nobuko ; Yasumura, Kohei ; Tomita, Ken Ichi ; Ihara, Kenji ; Fujii, Yoshimitsu ; Nakabeppu, Yusaku ; Sekiguchi, Mutsuo ; Fujii, Satoshi. / Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli. In: Cell. 1996 ; Vol. 86, No. 2. pp. 311-319.
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