Three envelope proteins of hepatitis B virus

Large S, middle S, and major S proteins needed for the formation of dane particles

Keiji Ueda, Toshiki Tsurimoto, Kenichi Matsubara

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The infectious particles of hepatitis B virus are called Dane particles and consist of viral nucleic acid encapsulated within a core particle that is enveloped by virus-coded surface proteins. The major S protein constitutes a significant fraction of these surface proteins. In addition, there are two other related proteins (large S and middle S), but their role in envelope formation has not yet been elucidated. We modified the translation initiation codon ATG of each of the envelope proteins by site-directed mutagenesis and found that mutant genomes that did not produce one or two of these proteins were unable to form Dane particles. The particles released into the culture medium by such mutants did not carry DNA. Synthesis of virus-coded RNA still occurred normally, and core particles carrying DNA accumulated intracellularly. The DNA in such core particles was mostly in the double-stranded open circular form, in contrast to the normal situation in which the particles contain mostly RNA and its complementary single-stranded DNA or else contain linear DNA that is partially single stranded and otherwise duplex. The role of the large S and middle S proteins in the formation of Dane particles is discussed.

Original languageEnglish
Pages (from-to)3521-3529
Number of pages9
JournalJournal of Virology
Volume65
Issue number7
Publication statusPublished - Jul 1991
Externally publishedYes

Fingerprint

Hepatitis B virus
Protein S
DNA
Membrane Proteins
proteins
Initiator Codon
Hepatitis A
Single-Stranded DNA
RNA Viruses
Site-Directed Mutagenesis
surface proteins
Nucleic Acids
Culture Media
Proteins
Complementary DNA
Genome
RNA
Viruses
hepatitis B virus S envelope protein
mutants

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Three envelope proteins of hepatitis B virus : Large S, middle S, and major S proteins needed for the formation of dane particles. / Ueda, Keiji; Tsurimoto, Toshiki; Matsubara, Kenichi.

In: Journal of Virology, Vol. 65, No. 7, 07.1991, p. 3521-3529.

Research output: Contribution to journalArticle

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