Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pemix: Interactions with the two DNA polymerases

Katsuya Daimon, Yutaka Kawarabayasi, Hisashi Kikuchi, Yoshihiko Sako, Yoshizumi Ishino

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Proliferating cell nuclear antigen (PCNA) is an essential component in the eukaryotic DNA replication machinery, in which it works for tethering DNA polymerases on the DNA template to accomplish processive DNA synthesis. The PCNA also interacts with many other proteins in important cellular processes, including cell cycle control, DNA repair, and an apoptotic pathway in the domain Eucarya. We identified three genes encoding PCNA-like sequences in the genome of Aeropyrum pernix, a crenarchaeal archaeon. We cloned and expressed these genes in Escherichia coli and analyzed the gene products. All three PCNA homologs stimulated the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II, identified in A. pernix to various extents, among which A. pernix PCNA 3 (ApePCNA3) provided a most remarkable effect on both Pol I and Pol II. The three proteins were confirmed to exist in the A. pernix cells. These results suggest that the three PCNAs work as the processivity factor of DNA polymerases in A. pernix cells under different conditions. In Eucarya, three checkpoint proteins, Hus1, Rad1, and Rad9, have been proposed to form a PCNA-like ring structure and may work as a sliding clamp for the translesion DNA polymerases. Therefore, it is very interesting that three active PCNAs were found in one archaeal cell. Further analyses are necessary to determine whether each PCNA has specific roles, and moreover, how they reveal different functions in the cells.

Original languageEnglish
Pages (from-to)687-694
Number of pages8
JournalJournal of bacteriology
Volume184
Issue number3
DOIs
Publication statusPublished - Jan 1 2002

Fingerprint

Aeropyrum
Archaea
Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
Proteins
Eukaryota
Genes
DNA
Cell Cycle Checkpoints
DNA Replication
DNA Repair

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pemix : Interactions with the two DNA polymerases. / Daimon, Katsuya; Kawarabayasi, Yutaka; Kikuchi, Hisashi; Sako, Yoshihiko; Ishino, Yoshizumi.

In: Journal of bacteriology, Vol. 184, No. 3, 01.01.2002, p. 687-694.

Research output: Contribution to journalArticle

@article{b19d8d8c5fb04eaca7fecce572fd1a51,
title = "Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pemix: Interactions with the two DNA polymerases",
abstract = "Proliferating cell nuclear antigen (PCNA) is an essential component in the eukaryotic DNA replication machinery, in which it works for tethering DNA polymerases on the DNA template to accomplish processive DNA synthesis. The PCNA also interacts with many other proteins in important cellular processes, including cell cycle control, DNA repair, and an apoptotic pathway in the domain Eucarya. We identified three genes encoding PCNA-like sequences in the genome of Aeropyrum pernix, a crenarchaeal archaeon. We cloned and expressed these genes in Escherichia coli and analyzed the gene products. All three PCNA homologs stimulated the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II, identified in A. pernix to various extents, among which A. pernix PCNA 3 (ApePCNA3) provided a most remarkable effect on both Pol I and Pol II. The three proteins were confirmed to exist in the A. pernix cells. These results suggest that the three PCNAs work as the processivity factor of DNA polymerases in A. pernix cells under different conditions. In Eucarya, three checkpoint proteins, Hus1, Rad1, and Rad9, have been proposed to form a PCNA-like ring structure and may work as a sliding clamp for the translesion DNA polymerases. Therefore, it is very interesting that three active PCNAs were found in one archaeal cell. Further analyses are necessary to determine whether each PCNA has specific roles, and moreover, how they reveal different functions in the cells.",
author = "Katsuya Daimon and Yutaka Kawarabayasi and Hisashi Kikuchi and Yoshihiko Sako and Yoshizumi Ishino",
year = "2002",
month = "1",
day = "1",
doi = "10.1128/JB.184.3.687-694.2002",
language = "English",
volume = "184",
pages = "687--694",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Three proliferating cell nuclear antigen-like proteins found in the hyperthermophilic archaeon Aeropyrum pemix

T2 - Interactions with the two DNA polymerases

AU - Daimon, Katsuya

AU - Kawarabayasi, Yutaka

AU - Kikuchi, Hisashi

AU - Sako, Yoshihiko

AU - Ishino, Yoshizumi

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Proliferating cell nuclear antigen (PCNA) is an essential component in the eukaryotic DNA replication machinery, in which it works for tethering DNA polymerases on the DNA template to accomplish processive DNA synthesis. The PCNA also interacts with many other proteins in important cellular processes, including cell cycle control, DNA repair, and an apoptotic pathway in the domain Eucarya. We identified three genes encoding PCNA-like sequences in the genome of Aeropyrum pernix, a crenarchaeal archaeon. We cloned and expressed these genes in Escherichia coli and analyzed the gene products. All three PCNA homologs stimulated the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II, identified in A. pernix to various extents, among which A. pernix PCNA 3 (ApePCNA3) provided a most remarkable effect on both Pol I and Pol II. The three proteins were confirmed to exist in the A. pernix cells. These results suggest that the three PCNAs work as the processivity factor of DNA polymerases in A. pernix cells under different conditions. In Eucarya, three checkpoint proteins, Hus1, Rad1, and Rad9, have been proposed to form a PCNA-like ring structure and may work as a sliding clamp for the translesion DNA polymerases. Therefore, it is very interesting that three active PCNAs were found in one archaeal cell. Further analyses are necessary to determine whether each PCNA has specific roles, and moreover, how they reveal different functions in the cells.

AB - Proliferating cell nuclear antigen (PCNA) is an essential component in the eukaryotic DNA replication machinery, in which it works for tethering DNA polymerases on the DNA template to accomplish processive DNA synthesis. The PCNA also interacts with many other proteins in important cellular processes, including cell cycle control, DNA repair, and an apoptotic pathway in the domain Eucarya. We identified three genes encoding PCNA-like sequences in the genome of Aeropyrum pernix, a crenarchaeal archaeon. We cloned and expressed these genes in Escherichia coli and analyzed the gene products. All three PCNA homologs stimulated the primer extension activities of the two DNA polymerases, polymerase I (Pol I) and Pol II, identified in A. pernix to various extents, among which A. pernix PCNA 3 (ApePCNA3) provided a most remarkable effect on both Pol I and Pol II. The three proteins were confirmed to exist in the A. pernix cells. These results suggest that the three PCNAs work as the processivity factor of DNA polymerases in A. pernix cells under different conditions. In Eucarya, three checkpoint proteins, Hus1, Rad1, and Rad9, have been proposed to form a PCNA-like ring structure and may work as a sliding clamp for the translesion DNA polymerases. Therefore, it is very interesting that three active PCNAs were found in one archaeal cell. Further analyses are necessary to determine whether each PCNA has specific roles, and moreover, how they reveal different functions in the cells.

UR - http://www.scopus.com/inward/record.url?scp=0036180382&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036180382&partnerID=8YFLogxK

U2 - 10.1128/JB.184.3.687-694.2002

DO - 10.1128/JB.184.3.687-694.2002

M3 - Article

C2 - 11790738

AN - SCOPUS:0036180382

VL - 184

SP - 687

EP - 694

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -