Thyrotropin decreases leptin production in rat adipocytes

Mitsuyo Shintani, Haruo Nishimura, Takashi Akamizu, Shin Yonemitsu, Hiroaki Masuzaki, Yoshihiro Ogawa, Kiminori Hosoda, Gen Inoue, Yasunao Yoshimasa, Kazuwa Nakao

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Leptin, which is secreted from adipocytes, has a role in the regulation of appetite and energy expenditure. The thyrotropin receptor (TSH-R) was recently found in adipocytes. We examined the effects of TSH on leptin production and lipolysis in rat epididymal adipocytes. TSH decreased the concentration of leptin in the medium time (~24 hours), and dose (~10-7 mol/L)-dependently (half-maximal inhibition [IC50] ≃ 10-9 mol/L). TSH also decreased the ob mRNA level approximately 55% in adipocytes. We confirmed the presence of TSH-R mRNA in the adipocytes by reverse transcription-polymerase chain reaction (RT-PCR). TSH stimulated glycerol release dose-dependently (IC50 ≃ 10-8 mol/L) in adipocytes. This TSH- induced glycerol release was further enhanced by adenosine deaminase (ADA). In summary, TSH reduced leptin production and stimulated lipolysis in rat epididymal adipocytes. Although the pathophysiological relevance of the regulation of leptin production and lipolysis by TSH is unknown, we speculate that TSH may affect the regulation of appetite and energy expenditure in pathophysiological states.

Original languageEnglish
Pages (from-to)1570-1574
Number of pages5
JournalMetabolism: Clinical and Experimental
Volume48
Issue number12
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

Thyrotropin
Leptin
Adipocytes
Lipolysis
Appetite Regulation
Glycerol
Energy Metabolism
Inhibitory Concentration 50
Thyrotropin Receptors
Messenger RNA
Adenosine Deaminase
Reverse Transcription
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Shintani, M., Nishimura, H., Akamizu, T., Yonemitsu, S., Masuzaki, H., Ogawa, Y., ... Nakao, K. (1999). Thyrotropin decreases leptin production in rat adipocytes. Metabolism: Clinical and Experimental, 48(12), 1570-1574. https://doi.org/10.1016/S0026-0495(99)90247-0

Thyrotropin decreases leptin production in rat adipocytes. / Shintani, Mitsuyo; Nishimura, Haruo; Akamizu, Takashi; Yonemitsu, Shin; Masuzaki, Hiroaki; Ogawa, Yoshihiro; Hosoda, Kiminori; Inoue, Gen; Yoshimasa, Yasunao; Nakao, Kazuwa.

In: Metabolism: Clinical and Experimental, Vol. 48, No. 12, 01.01.1999, p. 1570-1574.

Research output: Contribution to journalArticle

Shintani, M, Nishimura, H, Akamizu, T, Yonemitsu, S, Masuzaki, H, Ogawa, Y, Hosoda, K, Inoue, G, Yoshimasa, Y & Nakao, K 1999, 'Thyrotropin decreases leptin production in rat adipocytes', Metabolism: Clinical and Experimental, vol. 48, no. 12, pp. 1570-1574. https://doi.org/10.1016/S0026-0495(99)90247-0
Shintani M, Nishimura H, Akamizu T, Yonemitsu S, Masuzaki H, Ogawa Y et al. Thyrotropin decreases leptin production in rat adipocytes. Metabolism: Clinical and Experimental. 1999 Jan 1;48(12):1570-1574. https://doi.org/10.1016/S0026-0495(99)90247-0
Shintani, Mitsuyo ; Nishimura, Haruo ; Akamizu, Takashi ; Yonemitsu, Shin ; Masuzaki, Hiroaki ; Ogawa, Yoshihiro ; Hosoda, Kiminori ; Inoue, Gen ; Yoshimasa, Yasunao ; Nakao, Kazuwa. / Thyrotropin decreases leptin production in rat adipocytes. In: Metabolism: Clinical and Experimental. 1999 ; Vol. 48, No. 12. pp. 1570-1574.
@article{487ecb46e4a34feebf32ff4d2de74668,
title = "Thyrotropin decreases leptin production in rat adipocytes",
abstract = "Leptin, which is secreted from adipocytes, has a role in the regulation of appetite and energy expenditure. The thyrotropin receptor (TSH-R) was recently found in adipocytes. We examined the effects of TSH on leptin production and lipolysis in rat epididymal adipocytes. TSH decreased the concentration of leptin in the medium time (~24 hours), and dose (~10-7 mol/L)-dependently (half-maximal inhibition [IC50] ≃ 10-9 mol/L). TSH also decreased the ob mRNA level approximately 55{\%} in adipocytes. We confirmed the presence of TSH-R mRNA in the adipocytes by reverse transcription-polymerase chain reaction (RT-PCR). TSH stimulated glycerol release dose-dependently (IC50 ≃ 10-8 mol/L) in adipocytes. This TSH- induced glycerol release was further enhanced by adenosine deaminase (ADA). In summary, TSH reduced leptin production and stimulated lipolysis in rat epididymal adipocytes. Although the pathophysiological relevance of the regulation of leptin production and lipolysis by TSH is unknown, we speculate that TSH may affect the regulation of appetite and energy expenditure in pathophysiological states.",
author = "Mitsuyo Shintani and Haruo Nishimura and Takashi Akamizu and Shin Yonemitsu and Hiroaki Masuzaki and Yoshihiro Ogawa and Kiminori Hosoda and Gen Inoue and Yasunao Yoshimasa and Kazuwa Nakao",
year = "1999",
month = "1",
day = "1",
doi = "10.1016/S0026-0495(99)90247-0",
language = "English",
volume = "48",
pages = "1570--1574",
journal = "Metabolism: Clinical and Experimental",
issn = "0026-0495",
publisher = "W.B. Saunders Ltd",
number = "12",

}

TY - JOUR

T1 - Thyrotropin decreases leptin production in rat adipocytes

AU - Shintani, Mitsuyo

AU - Nishimura, Haruo

AU - Akamizu, Takashi

AU - Yonemitsu, Shin

AU - Masuzaki, Hiroaki

AU - Ogawa, Yoshihiro

AU - Hosoda, Kiminori

AU - Inoue, Gen

AU - Yoshimasa, Yasunao

AU - Nakao, Kazuwa

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Leptin, which is secreted from adipocytes, has a role in the regulation of appetite and energy expenditure. The thyrotropin receptor (TSH-R) was recently found in adipocytes. We examined the effects of TSH on leptin production and lipolysis in rat epididymal adipocytes. TSH decreased the concentration of leptin in the medium time (~24 hours), and dose (~10-7 mol/L)-dependently (half-maximal inhibition [IC50] ≃ 10-9 mol/L). TSH also decreased the ob mRNA level approximately 55% in adipocytes. We confirmed the presence of TSH-R mRNA in the adipocytes by reverse transcription-polymerase chain reaction (RT-PCR). TSH stimulated glycerol release dose-dependently (IC50 ≃ 10-8 mol/L) in adipocytes. This TSH- induced glycerol release was further enhanced by adenosine deaminase (ADA). In summary, TSH reduced leptin production and stimulated lipolysis in rat epididymal adipocytes. Although the pathophysiological relevance of the regulation of leptin production and lipolysis by TSH is unknown, we speculate that TSH may affect the regulation of appetite and energy expenditure in pathophysiological states.

AB - Leptin, which is secreted from adipocytes, has a role in the regulation of appetite and energy expenditure. The thyrotropin receptor (TSH-R) was recently found in adipocytes. We examined the effects of TSH on leptin production and lipolysis in rat epididymal adipocytes. TSH decreased the concentration of leptin in the medium time (~24 hours), and dose (~10-7 mol/L)-dependently (half-maximal inhibition [IC50] ≃ 10-9 mol/L). TSH also decreased the ob mRNA level approximately 55% in adipocytes. We confirmed the presence of TSH-R mRNA in the adipocytes by reverse transcription-polymerase chain reaction (RT-PCR). TSH stimulated glycerol release dose-dependently (IC50 ≃ 10-8 mol/L) in adipocytes. This TSH- induced glycerol release was further enhanced by adenosine deaminase (ADA). In summary, TSH reduced leptin production and stimulated lipolysis in rat epididymal adipocytes. Although the pathophysiological relevance of the regulation of leptin production and lipolysis by TSH is unknown, we speculate that TSH may affect the regulation of appetite and energy expenditure in pathophysiological states.

UR - http://www.scopus.com/inward/record.url?scp=13044300890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13044300890&partnerID=8YFLogxK

U2 - 10.1016/S0026-0495(99)90247-0

DO - 10.1016/S0026-0495(99)90247-0

M3 - Article

C2 - 10599990

AN - SCOPUS:13044300890

VL - 48

SP - 1570

EP - 1574

JO - Metabolism: Clinical and Experimental

JF - Metabolism: Clinical and Experimental

SN - 0026-0495

IS - 12

ER -