Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration

Shujie Wang, Takashi Watanabe, Kenji Matsuzawa, Akira Katsumi, Mai Kakeno, Toshinori Matsui, Feng Ye, Kazuhide Sato, Kiyoko Murase, Ikuko Sugiyama, Kazushi Kimura, Akira Mizoguchi, Mark H. Ginsberg, John G. Collard, Kozo Kaibuchi

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Abstract

Migrating cells acquire front-rear polarity with a leading edge and a trailing tail for directional movement. The Rac exchange factor Tiam1 participates in polarized cell migration with the PAR complex of PAR3, PAR6, and atypical protein kinase C. However, it remains largely unknown how Tiam1 is regulated and contributes to the establishment of polarity in migrating cells. We show here that Tiam1 interacts directly with talin, which binds and activates integrins to mediate their signaling. Tiam1 accumulated at adhesions in a manner dependent on talin and the PAR complex. The interactions of talin with Tiam1 and the PAR complex were required for adhesion-induced Rac1 activation, cell spreading, and migration toward integrin substrates. Furthermore, Tiam1 acted with talin to regulate adhesion turnover. Thus, we propose that Tiam1, with the PAR complex, binds to integrins through talin and, together with the PAR complex, thereby regulates Rac1 activity and adhesion turnover for polarized migration.

Original languageEnglish
Pages (from-to)331-345
Number of pages15
JournalJournal of Cell Biology
Volume199
Issue number2
DOIs
Publication statusPublished - Oct 15 2012

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Talin
Cell Movement
Integrins

All Science Journal Classification (ASJC) codes

  • Cell Biology

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Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration. / Wang, Shujie; Watanabe, Takashi; Matsuzawa, Kenji; Katsumi, Akira; Kakeno, Mai; Matsui, Toshinori; Ye, Feng; Sato, Kazuhide; Murase, Kiyoko; Sugiyama, Ikuko; Kimura, Kazushi; Mizoguchi, Akira; Ginsberg, Mark H.; Collard, John G.; Kaibuchi, Kozo.

In: Journal of Cell Biology, Vol. 199, No. 2, 15.10.2012, p. 331-345.

Research output: Contribution to journalArticle

Wang, S, Watanabe, T, Matsuzawa, K, Katsumi, A, Kakeno, M, Matsui, T, Ye, F, Sato, K, Murase, K, Sugiyama, I, Kimura, K, Mizoguchi, A, Ginsberg, MH, Collard, JG & Kaibuchi, K 2012, 'Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration', Journal of Cell Biology, vol. 199, no. 2, pp. 331-345. https://doi.org/10.1083/jcb.201202041
Wang, Shujie ; Watanabe, Takashi ; Matsuzawa, Kenji ; Katsumi, Akira ; Kakeno, Mai ; Matsui, Toshinori ; Ye, Feng ; Sato, Kazuhide ; Murase, Kiyoko ; Sugiyama, Ikuko ; Kimura, Kazushi ; Mizoguchi, Akira ; Ginsberg, Mark H. ; Collard, John G. ; Kaibuchi, Kozo. / Tiam1 interaction with the PAR complex promotes talin-mediated Rac1 activation during polarized cell migration. In: Journal of Cell Biology. 2012 ; Vol. 199, No. 2. pp. 331-345.
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