Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells

Tsuneyuki Tatsuke, Jae Man Lee, Takahiro Kusakabe, Kazuhiro Iiyama, Hideki Sezutsu, Keiro Uchino

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promoters for a Bombyx mori Tet-on system. In order to further improve this system, Giant, a transcriptional silencer from Drosophila melanogaster, is introduced to repress leaky transcription in the absence of doxycycline. Further, the promoter responsibility to the tetracycline-controlled transactivators is facilitated by introducing a synthetic minimal core promoter. With the tightly regulated second-generation silkworm Tet-on system, we obtain up to 300-fold induction of gene expression with the addition of doxycycline.

Original languageEnglish
Pages (from-to)173-182
Number of pages10
JournalArchives of insect biochemistry and physiology
Volume82
Issue number4
DOIs
Publication statusPublished - Apr 2013

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Insect Science

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