Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle

Mai Khoi Q. Do, Takahiro Suzuki, Borjigin Gerelt, Yusuke Sato, Wataru Mizunoya, Mako Nakamura, Yoshihide Ikeuchi, Judy E. Anderson, Ryuichi Tatsumi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8days post-crush, whereas active TGF-β3 increases after 12days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.

Original languageEnglish
Pages (from-to)712-717
Number of pages6
JournalAnimal Science Journal
Volume83
Issue number10
DOIs
Publication statusPublished - Oct 1 2012

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hepatocyte growth factor
transforming growth factors
Hepatocyte Growth Factor
Transforming Growth Factors
Fibroblast Growth Factor 2
Semaphorin-3A
skeletal muscle
Skeletal Muscle
physiological regulation
fibroblast growth factor 2
muscles
chemiluminescence
limbs (animal)
axons
animal injuries
Primary Cell Culture
in vitro studies
Neuromuscular Junction
Extracellular Fluid
Luminescence

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

Cite this

Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle. / Do, Mai Khoi Q.; Suzuki, Takahiro; Gerelt, Borjigin; Sato, Yusuke; Mizunoya, Wataru; Nakamura, Mako; Ikeuchi, Yoshihide; Anderson, Judy E.; Tatsumi, Ryuichi.

In: Animal Science Journal, Vol. 83, No. 10, 01.10.2012, p. 712-717.

Research output: Contribution to journalArticle

Do, Mai Khoi Q. ; Suzuki, Takahiro ; Gerelt, Borjigin ; Sato, Yusuke ; Mizunoya, Wataru ; Nakamura, Mako ; Ikeuchi, Yoshihide ; Anderson, Judy E. ; Tatsumi, Ryuichi. / Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle. In: Animal Science Journal. 2012 ; Vol. 83, No. 10. pp. 712-717.
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