Abstract
Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8days post-crush, whereas active TGF-β3 increases after 12days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 712-717 |
| Number of pages | 6 |
| Journal | Animal Science Journal |
| Volume | 83 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2012 |
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All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)
Cite this
Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle. / Do, Mai Khoi Q.; Suzuki, Takahiro; Gerelt, Borjigin; Sato, Yusuke; Mizunoya, Wataru; Nakamura, Mako; Ikeuchi, Yoshihide; Anderson, Judy E.; Tatsumi, Ryuichi.
In: Animal Science Journal, Vol. 83, No. 10, 01.10.2012, p. 712-717.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle
AU - Do, Mai Khoi Q.
AU - Suzuki, Takahiro
AU - Gerelt, Borjigin
AU - Sato, Yusuke
AU - Mizunoya, Wataru
AU - Nakamura, Mako
AU - Ikeuchi, Yoshihide
AU - Anderson, Judy E.
AU - Tatsumi, Ryuichi
PY - 2012/10/1
Y1 - 2012/10/1
N2 - Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8days post-crush, whereas active TGF-β3 increases after 12days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.
AB - Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon-guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early-differentiated satellite cells up-regulate a secreted neural chemorepellent Sema3A upon in vivo muscle-crush injury. The study also revealed that Sema3A expression is up-regulated in primary satellite-cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)-β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time-course of extracellular HGF, FGF2 and TGF-β3 concentrations after crush-injury of Gastrocnemius muscle in the rat lower hind-limb, using a combination of a non-homogenization/non-spin extraction of extracellular wound fluids and enhanced chemiluminescence-Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2-8days post-crush, whereas active TGF-β3 increases after 12days, providing a better understanding of the time-coordinated levels of HGF, FGF2 and TGF-β3 that drive regulation of Sema3A expression during regenerative intramuscular moto-neuritogenesis.
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UR - http://www.scopus.com/inward/citedby.url?scp=84867230512&partnerID=8YFLogxK
U2 - 10.1111/j.1740-0929.2012.01057.x
DO - 10.1111/j.1740-0929.2012.01057.x
M3 - Article
C2 - 23035711
AN - SCOPUS:84867230512
VL - 83
SP - 712
EP - 717
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 10
ER -