Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies

Tatsuya Okuda, Satoru Kidoaki, Mio Ohsaki, Yoshiyuki Koyama, Kenichi Yoshikawa, Takuro Niidome, Haruhiko Aoyagi

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

Dendritic poly(L-lysine) of the 6th generation shows high transfection efficiency into several cultivated cells with low cytotoxicity. In order to understand the mechanism of complex formation with plasmid DNA, the complex was observed using atomic force microscopy. After mixing for 15 min, 1-2 μm assemblies of complexes composed of several small particles (50-200 nm) were observed. At the same time, individual small complexes of 50 to 500 nm were observed on a mica surface. After incubation for 2 h, only the large complexes were found on the mica surface. As a result of further dynamic light scattering analysis and measurement of the transfection efficiency at different time points, the transfection efficiency of KG6 was found to increase with increasing size of the DNA-complexes. This result indicates that large complexes of more than 1 μm are major species that contribute to transfection in vitro.

Original languageEnglish
Pages (from-to)1270-1273
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume1
Issue number8
DOIs
Publication statusPublished - Apr 21 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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