Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies

Tatsuya Okuda, Satoru Kidoaki, Mio Ohsaki, Yoshiyuki Koyama, Kenichi Yoshikawa, Takuro Niidome, Haruhiko Aoyagi

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Dendritic poly(L-lysine) of the 6th generation shows high transfection efficiency into several cultivated cells with low cytotoxicity. In order to understand the mechanism of complex formation with plasmid DNA, the complex was observed using atomic force microscopy. After mixing for 15 min, 1-2 μm assemblies of complexes composed of several small particles (50-200 nm) were observed. At the same time, individual small complexes of 50 to 500 nm were observed on a mica surface. After incubation for 2 h, only the large complexes were found on the mica surface. As a result of further dynamic light scattering analysis and measurement of the transfection efficiency at different time points, the transfection efficiency of KG6 was found to increase with increasing size of the DNA-complexes. This result indicates that large complexes of more than 1 μm are major species that contribute to transfection in vitro.

Original languageEnglish
Pages (from-to)1270-1273
Number of pages4
JournalOrganic and Biomolecular Chemistry
Volume1
Issue number8
DOIs
Publication statusPublished - Apr 21 2003

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plasmids
lysine
Lysine
Transfection
Plasmids
deoxyribonucleic acid
mica
DNA
Dynamic light scattering
Cytotoxicity
assemblies
Atomic Force Microscopy
Atomic force microscopy
light scattering
atomic force microscopy
cells
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies. / Okuda, Tatsuya; Kidoaki, Satoru; Ohsaki, Mio; Koyama, Yoshiyuki; Yoshikawa, Kenichi; Niidome, Takuro; Aoyagi, Haruhiko.

In: Organic and Biomolecular Chemistry, Vol. 1, No. 8, 21.04.2003, p. 1270-1273.

Research output: Contribution to journalArticle

Okuda, Tatsuya ; Kidoaki, Satoru ; Ohsaki, Mio ; Koyama, Yoshiyuki ; Yoshikawa, Kenichi ; Niidome, Takuro ; Aoyagi, Haruhiko. / Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies. In: Organic and Biomolecular Chemistry. 2003 ; Vol. 1, No. 8. pp. 1270-1273.
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AU - Niidome, Takuro

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