TY - JOUR
T1 - Time-dependent increase of plasma cGMP concentration followed by oral EGCG administration in mice
AU - Tanaka, Yasutake
AU - Kumazoe, Motofumi
AU - Onda, Hiroaki
AU - Fujimura, Yoshinori
AU - Tachibana, Hirofumi
N1 - Funding Information:
This study was supported by Grants-in-Aid for Scientific Research of Japan Society for the Promotion of Science (JSPS KAKENHI) [Grant number JP 15H02448 , JP 18K14442 , JP 20H05683 ]. This study was technically supported by the Center for Advanced Instrumental and Educational Supports, Faculty of Agriculture , Kyushu University .
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/6
Y1 - 2021/6
N2 - Epigallocatechin 3-O-gallate (EGCG), the major catechin in green tea, exerts several beneficial effects in vivo. A previous study showed that cyclic guanosine monophosphate (cGMP) as a cellular second messenger has been shown to be an important mediator of the effects of EGCG. In general, long-term animal experiments are required to evaluate the functionality of phytochemicals such as EGCG. Thus, it might be beneficial to establish a marker for short-term evaluation of functional phytochemicals. In this study, the use of the plasma cGMP level was evaluated as a surrogate marker for the functional effectiveness of EGCG. Blood samples were collected from 6-wk-old C57BL/6J mice from 1 to 8 h after oral administration of EGCG (30 or 100 mg/kg). The upregulation of plasma cGMP levels elicited by the oral EGCG administration was evaluated. Additionally, it was confirmed that EGCG induced the expressions of intracellular Toll interacting protein (Tollip), which is mainly controlled by a cGMP signal in the spleen. These data indicated that the plasma cGMP levels could be a screening marker for the effect of EGCG.
AB - Epigallocatechin 3-O-gallate (EGCG), the major catechin in green tea, exerts several beneficial effects in vivo. A previous study showed that cyclic guanosine monophosphate (cGMP) as a cellular second messenger has been shown to be an important mediator of the effects of EGCG. In general, long-term animal experiments are required to evaluate the functionality of phytochemicals such as EGCG. Thus, it might be beneficial to establish a marker for short-term evaluation of functional phytochemicals. In this study, the use of the plasma cGMP level was evaluated as a surrogate marker for the functional effectiveness of EGCG. Blood samples were collected from 6-wk-old C57BL/6J mice from 1 to 8 h after oral administration of EGCG (30 or 100 mg/kg). The upregulation of plasma cGMP levels elicited by the oral EGCG administration was evaluated. Additionally, it was confirmed that EGCG induced the expressions of intracellular Toll interacting protein (Tollip), which is mainly controlled by a cGMP signal in the spleen. These data indicated that the plasma cGMP levels could be a screening marker for the effect of EGCG.
UR - http://www.scopus.com/inward/record.url?scp=85103344437&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85103344437&partnerID=8YFLogxK
U2 - 10.1016/j.fbio.2021.101017
DO - 10.1016/j.fbio.2021.101017
M3 - Article
AN - SCOPUS:85103344437
SN - 2212-4292
VL - 41
JO - Food Bioscience
JF - Food Bioscience
M1 - 101017
ER -