Time-Lapse Mechanical Characterization of Zona Pellucida Using a Cell Carrier Chip

Kou Nakahara, Shinya Sakuma, Manabu Kawahara, Masashi Takahashi, Fumihito Arai

Research output: Contribution to journalArticlepeer-review

Abstract

The aim of this paper is to measure the changes in the mechanical characteristics of the zona pellucida (ZP) of an oocyte over time. To measure the mechanical characteristics such as Young's modulus during cultivation, it is necessary to achieve the cultivation and the measurement of the mechanical characteristics of target oocytes. Generally, measurement systems are constructed on a microscope for observing the deformation of the oocytes, but it is difficult to integrate such a system with a conventional incubator. Therefore, we propose a cell carrier chip for the time-lapse mechanical characterization. The cell carrier chip has a probe as well as a force sensor to measure the mechanical characteristics of the ZP. In addition, the chip enables us to carry the target oocyte from the measurement system to the incubator. To calculate the Young's modulus of the ZP, we used an elastic spherical shell model and measured the Young's modulus of the ZP of cryopreserved oocytes at 0.5, 1, 2, 3, 4, 5, and 6 h after being thawed. The result indicates that the Young's modulus of the ZP tends to increase with the cultivation time. [2017-0133]

Original languageEnglish
Pages (from-to)464-471
Number of pages8
JournalJournal of Microelectromechanical Systems
Volume27
Issue number3
DOIs
Publication statusPublished - Jun 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Time-Lapse Mechanical Characterization of Zona Pellucida Using a Cell Carrier Chip'. Together they form a unique fingerprint.

Cite this