Time-lapse observation of cell adhesion by evanescent field imaging technique

Y. Arima, K. Kato, H. Iwata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The cell adhesion processes were analyzed using total internal reflection fluorescence microscope (TIRFM). It was observed that when the parallel beam of a light in a medium of lower refractive index strikes an interface with a medium of lower refractive index, it suffers total internal reflection when the angle of incidence exceeds the critical angle. It was found that after 1-hr incubation, the number of observed cells was almost constant, while the adhesion area slightly decreased with time of incubation, which suggested the morphological changes of adhered cells. The results demonstrated the utility of total internal reflection fluorescence microscope (TIRFM) to observe morphological change near the material surface.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - Dec 1 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: May 17 2004May 21 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period5/17/045/21/04

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All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Arima, Y., Kato, K., & Iwata, H. (2004). Time-lapse observation of cell adhesion by evanescent field imaging technique. In Transactions - 7th World Biomaterials Congress (Transactions - 7th World Biomaterials Congress).