Tissue-autonomous promotion of palisade cell development by phototropin 2 in Arabidopsis

Toshiaki Kozuka, Sam Geun Kong, Michio Doi, Ken Ichiro Shimazaki, Akira Nagatani

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Light is an important environmental information source that plants use to modify their growth and development. Palisade parenchyma cells in leaves develop cylindrical shapes in response to blue light; however, the photosensory mechanism for this response has not been elucidated. In this study, we analyzed the palisade cell response in phototropin-deficient mutants. First, we found that two different light-sensing mechanisms contributed to the response in different proportions depending on the light intensity. One response observed under lower intensities of blue light was mediated exclusively by a blue light photoreceptor, phototropin 2 (PHOT2). Another response was elicited under higher intensities of light in a phototropin-independent manner. To determine the tissue in which PHOT2 perceives the light stimulus to regulate the response, green fluorescent protein (GFP)-tagged PHOT2 (P2G) was expressed under the control of tissue-specific promoters in the phot1 phot2 mutant background. The results revealed that the expression of P2G in the mesophyll, but not in the epidermis, promoted palisade cell development. Furthermore, a constitutively active C-terminal kinase fragment of PHOT2 fused to GFP (P2CG) promoted the development of cylindrical palisade cells in the proper direction without the directional cue provided by light. Hence, in response to blue light, PHOT2 promotes the development of cylindrical palisade cells along a predetermined axis in a tissue-autonomous manner.

Original languageEnglish
Pages (from-to)3684-3695
Number of pages12
JournalPlant Cell
Volume23
Issue number10
DOIs
Publication statusPublished - Dec 1 2011

Fingerprint

Phototropins
Arabidopsis
blue light
Light
green fluorescent protein
cells
light intensity
mutants
epidermis (plant)
information sources
Green Fluorescent Proteins
photoreceptors
mesophyll
parenchyma (plant tissue)
growth and development
phosphotransferases (kinases)
promoter regions
tissues
Mesophyll Cells
Growth and Development

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Kozuka, T., Kong, S. G., Doi, M., Shimazaki, K. I., & Nagatani, A. (2011). Tissue-autonomous promotion of palisade cell development by phototropin 2 in Arabidopsis. Plant Cell, 23(10), 3684-3695. https://doi.org/10.1105/tpc.111.085852

Tissue-autonomous promotion of palisade cell development by phototropin 2 in Arabidopsis. / Kozuka, Toshiaki; Kong, Sam Geun; Doi, Michio; Shimazaki, Ken Ichiro; Nagatani, Akira.

In: Plant Cell, Vol. 23, No. 10, 01.12.2011, p. 3684-3695.

Research output: Contribution to journalArticle

Kozuka, T, Kong, SG, Doi, M, Shimazaki, KI & Nagatani, A 2011, 'Tissue-autonomous promotion of palisade cell development by phototropin 2 in Arabidopsis', Plant Cell, vol. 23, no. 10, pp. 3684-3695. https://doi.org/10.1105/tpc.111.085852
Kozuka, Toshiaki ; Kong, Sam Geun ; Doi, Michio ; Shimazaki, Ken Ichiro ; Nagatani, Akira. / Tissue-autonomous promotion of palisade cell development by phototropin 2 in Arabidopsis. In: Plant Cell. 2011 ; Vol. 23, No. 10. pp. 3684-3695.
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