Tissue distribution and subcellular localization of rabbit liver metalloendopeptidase

Kazunori Nakagawa, Shun Ichiro Kawabata, Yutaka Nakashima, Sadaaki Iwanaga, Katsuo Sueishi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have previously isolated rabbit liver microsomal metalloendopeptidase (MEP) as a candidate for the processing enzyme of vitamin K-dependent plasma proteins. A cDNA coding for MEP has revealed that it is structurally related to metalloendopeptidase-24.15, which catalyzes the proteolytic processing of several bioactive peptides. In this study we examined the tissue distribution and subcellular localization of MEP by light and electron microscopic immunohistochemical methods, in addition to Northern blot analysis. Chicken polyclonal antibodies were raised by using synthetic peptides AG1 (Met31- Asn46) and AG3 (Asp537-Gly551) derived from the sequence of MEP. Both anti-AG1 and anti-AG3 antibodies reacted specifically with MEP, as judged by Western blotting and immunohistochemical methods. Both antibodies gave an identical staining distribution, which was localized on the luminal cell surfaces and in the cytoplasm of the following organs: liver, brain, lungs, kidneys, esophagus, stomach, duodenum, pancreas, placenta, epididymis, uterus, ovary, and oviduct. Northern blot analysis revealed that the expression of MEP mRNA is similar to its immunohistochemical distribution except in the heart. These results suggest that MEP may participate more closely in a degradation role in peptide metabolism in various tissues than in a processing role of the proprotein, like metalloendopeptidase-24.15.

Original languageEnglish
Pages (from-to)41-47
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 1997

Fingerprint

Metalloendopeptidases
Tissue Distribution
Rabbits
Liver
thimet oligopeptidase
Northern Blotting
Peptides
Oviducts
Vitamin K
Antibodies
Epididymis
Duodenum
Placenta
Esophagus
Uterus
Blood Proteins
Pancreas
Anti-Idiotypic Antibodies
Ovary
Chickens

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Tissue distribution and subcellular localization of rabbit liver metalloendopeptidase. / Nakagawa, Kazunori; Kawabata, Shun Ichiro; Nakashima, Yutaka; Iwanaga, Sadaaki; Sueishi, Katsuo.

In: Journal of Histochemistry and Cytochemistry, Vol. 45, No. 1, 01.1997, p. 41-47.

Research output: Contribution to journalArticle

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