Tissue Inhibitor of Metalloproteinase 2 and Coronary Artery Lesions in Kawasaki Disease

Kenji Furuno, Hidetoshi Takada, Ken Yamamoto, Kazuyuki Ikeda, Takuro Ohno, Vahid Khajoee, Yumi Mizuno, Toshiro Hara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Objective: To identify cytokine genes uniquely expressed in peripheral blood mononuclear cells (PBMNCs) in the acute phase of Kawasaki disease (KD) with coronary artery lesions (CALs). Study design: We screened the mRNA expression levels of PBMNCs from 4 pairs of KD patients with and without CAL using DNA microarray. The result was confirmed by real-time polymerase chain reaction (RT-PCR). The genetic association study was performed to analyze the significance of single nucleotide polymorphisms in the identified gene for the development of CAL in KD patients (184 controls, 144 KD patients with CAL, 64 KD patients without CAL). Results: The microarray analysis showed that tissue inhibitor of metalloproteinases 2 (TIMP2) was expressed at higher levels in PBMNCs of KD patients with CAL than in KD patients without CAL. Quantitative RT-PCR confirmed that the expression levels were significantly higher in the KD patients with CAL than in those without CAL (P < .05). Among KD patients, TIMP2 promoter polymorphisms were significantly associated with a risk of CAL (P < .01). There was a significant difference in the transcriptional activities between the haplotypes of the TIMP2 promoter polymorphisms by reporter assay using U-937. Conclusions: TIMP2 overexpression and the promoter polymorphisms might play a role in the development of CALs.

Original languageEnglish
Pages (from-to)155-160.e1
JournalJournal of Pediatrics
Volume151
Issue number2
DOIs
Publication statusPublished - Aug 2007

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Tissue Inhibitor of Metalloproteinase-2
Mucocutaneous Lymph Node Syndrome
Coronary Vessels
Blood Cells
Real-Time Polymerase Chain Reaction
Genetic Association Studies
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Haplotypes
Genes
Single Nucleotide Polymorphism

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Furuno, K., Takada, H., Yamamoto, K., Ikeda, K., Ohno, T., Khajoee, V., ... Hara, T. (2007). Tissue Inhibitor of Metalloproteinase 2 and Coronary Artery Lesions in Kawasaki Disease. Journal of Pediatrics, 151(2), 155-160.e1. https://doi.org/10.1016/j.jpeds.2007.03.010

Tissue Inhibitor of Metalloproteinase 2 and Coronary Artery Lesions in Kawasaki Disease. / Furuno, Kenji; Takada, Hidetoshi; Yamamoto, Ken; Ikeda, Kazuyuki; Ohno, Takuro; Khajoee, Vahid; Mizuno, Yumi; Hara, Toshiro.

In: Journal of Pediatrics, Vol. 151, No. 2, 08.2007, p. 155-160.e1.

Research output: Contribution to journalArticle

Furuno, K, Takada, H, Yamamoto, K, Ikeda, K, Ohno, T, Khajoee, V, Mizuno, Y & Hara, T 2007, 'Tissue Inhibitor of Metalloproteinase 2 and Coronary Artery Lesions in Kawasaki Disease', Journal of Pediatrics, vol. 151, no. 2, pp. 155-160.e1. https://doi.org/10.1016/j.jpeds.2007.03.010
Furuno, Kenji ; Takada, Hidetoshi ; Yamamoto, Ken ; Ikeda, Kazuyuki ; Ohno, Takuro ; Khajoee, Vahid ; Mizuno, Yumi ; Hara, Toshiro. / Tissue Inhibitor of Metalloproteinase 2 and Coronary Artery Lesions in Kawasaki Disease. In: Journal of Pediatrics. 2007 ; Vol. 151, No. 2. pp. 155-160.e1.
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