Tissue-specific and light-responsive regulation of the promoter region of the Arabidopsis thaliana chloroplast ω-3 fatty acid desaturase gene (FAD7)

Takumi Nishiuchi, Takiko Nakamura, Tsuyoshi Abe, Hiroaki Kodama, Mitsuo Nishimura, Koh Iba

Research output: Contribution to journalArticle

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Abstract

The Arabidopsis FAD7 gene encodes a chloroplast ω-3 fatty acid desaturase that catalyzes the desaturation of lipid-linked dienoic fatty acids (18:2 and 16:2). An 825 bp FAD7 promoter fragment upstream from the transcriptional start point contained several short sequences which were homologous to the cis-elements (box II, G-box, etc.) conserved in many light-responsive genes. We introduced the FAD7 promoter fused to the β-glucuronidase (GUS) or the luciferase (LUC) reporter gene into tobacco plants. The -825 promoter sequence conferred tissue-specific and light-responsive expression to both these reporter genes in transgenic tobacco, indicating that these expressions of the FAD7 gene were regulated mainly at the transcriptional level. Histochemical GUS staining showed that the activity of the FAD7 promoter is restricted to the tissues with chloroplast-containing cells although the staining was noticeably absent in the chloroplast-containing cells associated with vascular systems. The 5′ deletion experiments of the promoter revealed that the -362/ -166 region, containing two putative box II sequences, was responsible for the tissue-specific and light-responsive expression of the FAD7 gene.

Original languageEnglish
Pages (from-to)599-609
Number of pages11
JournalPlant Molecular Biology
Volume29
Issue number3
DOIs
Publication statusPublished - Nov 1 1995

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Fatty Acid Desaturases
stearoyl-CoA desaturase
Chloroplasts
Arabidopsis
Genetic Promoter Regions
Arabidopsis thaliana
chloroplasts
Glucuronidase
promoter regions
Reporter Genes
Light
Tobacco
Staining and Labeling
Genes
Gene Expression
genes
reporter genes
Luciferases
tobacco
dienoic fatty acids

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Tissue-specific and light-responsive regulation of the promoter region of the Arabidopsis thaliana chloroplast ω-3 fatty acid desaturase gene (FAD7). / Nishiuchi, Takumi; Nakamura, Takiko; Abe, Tsuyoshi; Kodama, Hiroaki; Nishimura, Mitsuo; Iba, Koh.

In: Plant Molecular Biology, Vol. 29, No. 3, 01.11.1995, p. 599-609.

Research output: Contribution to journalArticle

Nishiuchi, Takumi ; Nakamura, Takiko ; Abe, Tsuyoshi ; Kodama, Hiroaki ; Nishimura, Mitsuo ; Iba, Koh. / Tissue-specific and light-responsive regulation of the promoter region of the Arabidopsis thaliana chloroplast ω-3 fatty acid desaturase gene (FAD7). In: Plant Molecular Biology. 1995 ; Vol. 29, No. 3. pp. 599-609.
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