Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1

Seicho Makihira, Takahiro Shuto, Hiroki Nikawa, Keishi Okamoto, Yuichi Mine, Yuko Takamoto, Masaru Ohara, Koichiro Tsuji

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration.

Original languageEnglish
Pages (from-to)1458-1470
Number of pages13
JournalInternational journal of molecular sciences
Volume11
Issue number4
DOIs
Publication statusPublished - Apr 1 2010

Fingerprint

Histatins
Porphyromonas gingivalis
Titanium
cultured cells
Peptides
peptides
titanium
Antimicrobial Cationic Peptides
Cells
Osseointegration
Cell Line
biofilms
Biofilms
Cell Differentiation
Messenger RNA
cells

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1. / Makihira, Seicho; Shuto, Takahiro; Nikawa, Hiroki; Okamoto, Keishi; Mine, Yuichi; Takamoto, Yuko; Ohara, Masaru; Tsuji, Koichiro.

In: International journal of molecular sciences, Vol. 11, No. 4, 01.04.2010, p. 1458-1470.

Research output: Contribution to journalArticle

Makihira, Seicho ; Shuto, Takahiro ; Nikawa, Hiroki ; Okamoto, Keishi ; Mine, Yuichi ; Takamoto, Yuko ; Ohara, Masaru ; Tsuji, Koichiro. / Titanium immobilized with an antimicrobial peptide derived from histatin accelerates the differentiation of osteoblastic cell line, MC3T3-E1. In: International journal of molecular sciences. 2010 ; Vol. 11, No. 4. pp. 1458-1470.
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