Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells

Seicho Makihira, Yuichi Mine, Hiroki Nikawa, Takahiro Shuto, Satoshi Iwata, Ryuji Hosokawa, Kohei Kamoi, Shota Okazaki, Yu Yamaguchi

Research output: Contribution to journalArticle

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Abstract

Gingival epithelial-like cells (GE-1) were cultured and used to examine the cellular responses of gingival tissues to varying concentrations of titanium (Ti) ions. Titanium ions at concentrations of more than 13. ppm significantly decreased the viability of GE-1 cells and increased LDH release from the cells into the supernatant, but had no significant effect on their caspase 3 activity. These data suggest that a high concentration of Ti ions induced necrosis of the GE-1 cells. Titanium ions at a concentration of 5. ppm significantly increased the level of CCL2 mRNA expression in GE-1 cells exposed to lipopolysaccharide derived from Porphyromonas gingivalis in a synergistic manner. Moreover, the mRNA expression levels of TLR-4 and ICAM-1 in GE-1 cells loaded with Ti ions at 9. ppm were significantly enhanced as compared with those in GE-1 cells without Ti stimulation. We suggest that Ti ions are in part responsible for monocyte infiltration in the oral cavity by elevating the sensitivity of gingival epithelial cells to microorganisms. Taken together, these data indicate that Ti ions may be involved in cytotoxicity and inflammation at the interfaces of dental implants and gingival tissue.

Original languageEnglish
Pages (from-to)1905-1910
Number of pages6
JournalToxicology in Vitro
Volume24
Issue number7
DOIs
Publication statusPublished - Oct 1 2010

Fingerprint

Titanium
Lipopolysaccharides
Necrosis
Epithelial Cells
Ions
Tissue
Porphyromonas gingivalis
Messenger RNA
Dental prostheses
Dental Implants
Intercellular Adhesion Molecule-1
Cytotoxicity
Infiltration
Caspase 3
Microorganisms
Mouth
Monocytes
Inflammation

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Makihira, S., Mine, Y., Nikawa, H., Shuto, T., Iwata, S., Hosokawa, R., ... Yamaguchi, Y. (2010). Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells. Toxicology in Vitro, 24(7), 1905-1910. https://doi.org/10.1016/j.tiv.2010.07.023

Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells. / Makihira, Seicho; Mine, Yuichi; Nikawa, Hiroki; Shuto, Takahiro; Iwata, Satoshi; Hosokawa, Ryuji; Kamoi, Kohei; Okazaki, Shota; Yamaguchi, Yu.

In: Toxicology in Vitro, Vol. 24, No. 7, 01.10.2010, p. 1905-1910.

Research output: Contribution to journalArticle

Makihira, S, Mine, Y, Nikawa, H, Shuto, T, Iwata, S, Hosokawa, R, Kamoi, K, Okazaki, S & Yamaguchi, Y 2010, 'Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells', Toxicology in Vitro, vol. 24, no. 7, pp. 1905-1910. https://doi.org/10.1016/j.tiv.2010.07.023
Makihira, Seicho ; Mine, Yuichi ; Nikawa, Hiroki ; Shuto, Takahiro ; Iwata, Satoshi ; Hosokawa, Ryuji ; Kamoi, Kohei ; Okazaki, Shota ; Yamaguchi, Yu. / Titanium ion induces necrosis and sensitivity to lipopolysaccharide in gingival epithelial-like cells. In: Toxicology in Vitro. 2010 ; Vol. 24, No. 7. pp. 1905-1910.
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