Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice

Hideyuki Shimizu, Tetsuya Matsuguchi, Yoshihide Fukuda, Isao Nakano, Tetsuo Hayakawa, Osamu Takeuchi, Shizuo Akira, Masayuki Umemura, Takashi Suda, Yasunobu Yoshikai

    Research output: Contribution to journalArticlepeer-review

    39 Citations (Scopus)

    Abstract

    Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.

    Original languageEnglish
    Pages (from-to)1265-1277
    Number of pages13
    JournalGastroenterology
    Volume123
    Issue number4
    DOIs
    Publication statusPublished - Oct 2002

    All Science Journal Classification (ASJC) codes

    • Hepatology
    • Gastroenterology

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