Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice

Hideyuki Shimizu, Tetsuya Matsuguchi, Yoshihide Fukuda, Isao Nakano, Tetsuo Hayakawa, Osamu Takeuchi, Shizuo Akira, Masayuki Umemura, Takashi Suda, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.

    Original languageEnglish
    Pages (from-to)1265-1277
    Number of pages13
    JournalGastroenterology
    Volume123
    Issue number4
    DOIs
    Publication statusPublished - Jan 1 2002

    Fingerprint

    Toll-Like Receptor 2
    Natural Killer T-Cells
    Fas Ligand Protein
    Salmonella Infections
    Liver
    Wounds and Injuries
    Toll-Like Receptors
    Alanine Transaminase
    Salmonella
    T-Lymphocyte Subsets
    Phagocytes
    Serum
    Reverse Transcription
    Flow Cytometry
    Enzyme-Linked Immunosorbent Assay
    Lymphocytes
    Gene Expression
    Polymerase Chain Reaction
    Infection
    Genes

    All Science Journal Classification (ASJC) codes

    • Hepatology
    • Gastroenterology

    Cite this

    Shimizu, H., Matsuguchi, T., Fukuda, Y., Nakano, I., Hayakawa, T., Takeuchi, O., ... Yoshikai, Y. (2002). Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice. Gastroenterology, 123(4), 1265-1277. https://doi.org/10.1053/gast.2002.36006

    Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice. / Shimizu, Hideyuki; Matsuguchi, Tetsuya; Fukuda, Yoshihide; Nakano, Isao; Hayakawa, Tetsuo; Takeuchi, Osamu; Akira, Shizuo; Umemura, Masayuki; Suda, Takashi; Yoshikai, Yasunobu.

    In: Gastroenterology, Vol. 123, No. 4, 01.01.2002, p. 1265-1277.

    Research output: Contribution to journalArticle

    Shimizu, H, Matsuguchi, T, Fukuda, Y, Nakano, I, Hayakawa, T, Takeuchi, O, Akira, S, Umemura, M, Suda, T & Yoshikai, Y 2002, 'Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice', Gastroenterology, vol. 123, no. 4, pp. 1265-1277. https://doi.org/10.1053/gast.2002.36006
    Shimizu, Hideyuki ; Matsuguchi, Tetsuya ; Fukuda, Yoshihide ; Nakano, Isao ; Hayakawa, Tetsuo ; Takeuchi, Osamu ; Akira, Shizuo ; Umemura, Masayuki ; Suda, Takashi ; Yoshikai, Yasunobu. / Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice. In: Gastroenterology. 2002 ; Vol. 123, No. 4. pp. 1265-1277.
    @article{27c6cb6044d04bf282ff8e3719f4021c,
    title = "Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice",
    abstract = "Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.",
    author = "Hideyuki Shimizu and Tetsuya Matsuguchi and Yoshihide Fukuda and Isao Nakano and Tetsuo Hayakawa and Osamu Takeuchi and Shizuo Akira and Masayuki Umemura and Takashi Suda and Yasunobu Yoshikai",
    year = "2002",
    month = "1",
    day = "1",
    doi = "10.1053/gast.2002.36006",
    language = "English",
    volume = "123",
    pages = "1265--1277",
    journal = "Gastroenterology",
    issn = "0016-5085",
    publisher = "W.B. Saunders Ltd",
    number = "4",

    }

    TY - JOUR

    T1 - Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice

    AU - Shimizu, Hideyuki

    AU - Matsuguchi, Tetsuya

    AU - Fukuda, Yoshihide

    AU - Nakano, Isao

    AU - Hayakawa, Tetsuo

    AU - Takeuchi, Osamu

    AU - Akira, Shizuo

    AU - Umemura, Masayuki

    AU - Suda, Takashi

    AU - Yoshikai, Yasunobu

    PY - 2002/1/1

    Y1 - 2002/1/1

    N2 - Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.

    AB - Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.

    UR - http://www.scopus.com/inward/record.url?scp=0036789130&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0036789130&partnerID=8YFLogxK

    U2 - 10.1053/gast.2002.36006

    DO - 10.1053/gast.2002.36006

    M3 - Article

    C2 - 12360487

    AN - SCOPUS:0036789130

    VL - 123

    SP - 1265

    EP - 1277

    JO - Gastroenterology

    JF - Gastroenterology

    SN - 0016-5085

    IS - 4

    ER -