Abstract
Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1265-1277 |
| Number of pages | 13 |
| Journal | Gastroenterology |
| Volume | 123 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 2002 |
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All Science Journal Classification (ASJC) codes
- Hepatology
- Gastroenterology
Cite this
Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice. / Shimizu, Hideyuki; Matsuguchi, Tetsuya; Fukuda, Yoshihide; Nakano, Isao; Hayakawa, Tetsuo; Takeuchi, Osamu; Akira, Shizuo; Umemura, Masayuki; Suda, Takashi; Yoshikai, Yasunobu.
In: Gastroenterology, Vol. 123, No. 4, 01.01.2002, p. 1265-1277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toll-like receptor 2 contributes to liver injury by Salmonella infection through Fas ligand expression on NKT cells in mice
AU - Shimizu, Hideyuki
AU - Matsuguchi, Tetsuya
AU - Fukuda, Yoshihide
AU - Nakano, Isao
AU - Hayakawa, Tetsuo
AU - Takeuchi, Osamu
AU - Akira, Shizuo
AU - Umemura, Masayuki
AU - Suda, Takashi
AU - Yoshikai, Yasunobu
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.
AB - Background & Aims: Toll-like receptors (TLRs) for bacterial constitutes are expressed not only by phagocytes but also by some subsets of T cells. We previously reported that natural killer T cells (NKT cells) play an important role in liver injury induced by Salmonella infection. In the present study, we investigated whether TLRs on NKT cells are involved in Salmonella-induced liver injury. Methods: Gene expression of TLR2 was examined in sorted natural killer, NKT, and T cells from livers of naive mice by the reverse-transcription polymerase chain reaction method. Serum alanine aminotransferase level and FasL expression on liver lymphocytes were examined in TLR2-deficient (TLR2-/-) and FasL-deficient gld/gld mice before and after intraperitoneal inoculation of Salmonella choleraesuis 31N-1 using an enzyme-linked immunosorbent assay and flow cytometry. Results: TLR2 gene was abundantly expressed by NKT cells freshly isolated from naive mice. FasL expression on liver NKT cells increased in TLR2+/- mice but not in TLR2-/- mice after Salmonella infection. Serum alanine aminotransferase level was significantly lower in the TLR2-/- and gld/gld mice than in the control mice after infection. Conclusions: TLR2 may contribute to liver injury induced by Salmonella infection via FasL induction on liver NKT cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036789130&partnerID=8YFLogxK
U2 - 10.1053/gast.2002.36006
DO - 10.1053/gast.2002.36006
M3 - Article
C2 - 12360487
AN - SCOPUS:0036789130
VL - 123
SP - 1265
EP - 1277
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 4
ER -