Toll-like receptor 2 is at least partly involved in the antitumor activity of glycoprotein from Chlorella vulgaris

Takashi Hasegawa, Tetsuya Matsuguchi, Kiyoshi Noda, Kuniaki Tanaka, Shoichiro Kumamoto, Yukihiro Shoyama, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    41 Citations (Scopus)

    Abstract

    Toll-like receptors (TLR) are involved in innate immunity by recognizing various bacterial components. We have previously reported that an active substance of ARS-2 purified from the culture medium of Chlorella vulgaris was a glycoprotein with a molecular weight of 63,100 amu and that this glycoprotein expressed antitumor activity, with the protein moiety in ARS-2 being necessary for this antitumor activity. Here, we show that ARS-2 stimulated spleen-adherent cells from C3H/HeJ lacking functional TLR4 to produce interleukin-12 (IL-12) p40, whereas such cytokine production was significantly impaired in ARS-2-stimulated spleen-adherent cells from TLR2 knockout mice. The overexpression of mouse TLR2 (mTLR2) and mouse CD14 (mCD14) conferred the ARS-2 inducibility of nuclear factor-κB activation to human HEK 293 cells. These results suggest that TLR2 signaling is at least partly involved in the antitumor activity of the water-soluble antitumor glycoprotein from C. vulgaris.

    Original languageEnglish
    Pages (from-to)579-589
    Number of pages11
    JournalInternational Immunopharmacology
    Volume2
    Issue number4
    DOIs
    Publication statusPublished - Mar 5 2002

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology
    • Pharmacology

    Fingerprint Dive into the research topics of 'Toll-like receptor 2 is at least partly involved in the antitumor activity of glycoprotein from Chlorella vulgaris'. Together they form a unique fingerprint.

  • Cite this