TY - JOUR
T1 - Toll-like receptor 2 is at least partly involved in the antitumor activity of glycoprotein from Chlorella vulgaris
AU - Hasegawa, Takashi
AU - Matsuguchi, Tetsuya
AU - Noda, Kiyoshi
AU - Tanaka, Kuniaki
AU - Kumamoto, Shoichiro
AU - Shoyama, Yukihiro
AU - Yoshikai, Yasunobu
PY - 2002
Y1 - 2002
N2 - Toll-like receptors (TLR) are involved in innate immunity by recognizing various bacterial components. We have previously reported that an active substance of ARS-2 purified from the culture medium of Chlorella vulgaris was a glycoprotein with a molecular weight of 63,100 amu and that this glycoprotein expressed antitumor activity, with the protein moiety in ARS-2 being necessary for this antitumor activity. Here, we show that ARS-2 stimulated spleen-adherent cells from C3H/HeJ lacking functional TLR4 to produce interleukin-12 (IL-12) p40, whereas such cytokine production was significantly impaired in ARS-2-stimulated spleen-adherent cells from TLR2 knockout mice. The overexpression of mouse TLR2 (mTLR2) and mouse CD14 (mCD14) conferred the ARS-2 inducibility of nuclear factor-κB activation to human HEK 293 cells. These results suggest that TLR2 signaling is at least partly involved in the antitumor activity of the water-soluble antitumor glycoprotein from C. vulgaris.
AB - Toll-like receptors (TLR) are involved in innate immunity by recognizing various bacterial components. We have previously reported that an active substance of ARS-2 purified from the culture medium of Chlorella vulgaris was a glycoprotein with a molecular weight of 63,100 amu and that this glycoprotein expressed antitumor activity, with the protein moiety in ARS-2 being necessary for this antitumor activity. Here, we show that ARS-2 stimulated spleen-adherent cells from C3H/HeJ lacking functional TLR4 to produce interleukin-12 (IL-12) p40, whereas such cytokine production was significantly impaired in ARS-2-stimulated spleen-adherent cells from TLR2 knockout mice. The overexpression of mouse TLR2 (mTLR2) and mouse CD14 (mCD14) conferred the ARS-2 inducibility of nuclear factor-κB activation to human HEK 293 cells. These results suggest that TLR2 signaling is at least partly involved in the antitumor activity of the water-soluble antitumor glycoprotein from C. vulgaris.
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U2 - 10.1016/S1567-5769(02)00002-4
DO - 10.1016/S1567-5769(02)00002-4
M3 - Article
C2 - 11962736
AN - SCOPUS:0036175944
SN - 1567-5769
VL - 2
SP - 579
EP - 589
JO - International Immunopharmacology
JF - International Immunopharmacology
IS - 4
ER -