Tom70 Is Essential for PINK1 Import into Mitochondria

Hiroki Kato, Qiping Lu, Doron Rapaport, Vera Kozjak-Pavlovic

Research output: Contribution to journalArticle

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Abstract

PTEN induced kinase 1 (PINK1) is a serine/threonine kinase in the outer membrane of mitochondria (OMM), and known as a responsible gene of Parkinson's disease (PD). The precursor of PINK1 is synthesized in the cytosol and then imported into the mitochondria via the translocase of the OMM (TOM) complex. However, a large part of PINK1 import mechanism remains unclear. In this study, we examined using cell-free system the mechanism by which PINK1 is targeted to and assembled into mitochondria. Surprisingly, the main component of the import channel, Tom40 was not necessary for PINK1 import. Furthermore, we revealed that the import receptor Tom70 is essential for PINK1 import. In addition, we observed that although PINK1 has predicted mitochondrial targeting signal, it was not processed by the mitochondrial processing peptidase. Thus, our results suggest that PINK1 is imported into mitochondria by a unique pathway that is independent of the TOM core complex but crucially depends on the import receptor Tom70.

Original languageEnglish
Article numbere58435
JournalPloS one
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 5 2013

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Mitochondria
imports
phosphotransferases (kinases)
mitochondria
Phosphotransferases
Membranes
Cell-Free System
cell free system
Protein-Serine-Threonine Kinases
receptors
Parkinson disease
peptidases
Cytosol
Parkinson Disease
threonine
cytosol
serine
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Tom70 Is Essential for PINK1 Import into Mitochondria. / Kato, Hiroki; Lu, Qiping; Rapaport, Doron; Kozjak-Pavlovic, Vera.

In: PloS one, Vol. 8, No. 3, e58435, 05.03.2013.

Research output: Contribution to journalArticle

Kato, H, Lu, Q, Rapaport, D & Kozjak-Pavlovic, V 2013, 'Tom70 Is Essential for PINK1 Import into Mitochondria', PloS one, vol. 8, no. 3, e58435. https://doi.org/10.1371/journal.pone.0058435
Kato, Hiroki ; Lu, Qiping ; Rapaport, Doron ; Kozjak-Pavlovic, Vera. / Tom70 Is Essential for PINK1 Import into Mitochondria. In: PloS one. 2013 ; Vol. 8, No. 3.
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