TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis

Takamasa Suzuki, Sakiko Nakajima, Soichi Inagaki, Makiko Hirano-Nakakita, Ken Matsuoka, Taku Demura, Hiroo Fukuda, Atsushi Morikami, Kenzo Nakamura

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

TONSOKU(TSK)/MGOUN3/BRUSHY1 of Arabidopsis thaliana encodes a nuclear leucine-glycine-aspargine (LGN) domain protein implicated to be involved in genome maintenance, and mutants with defects in TSK show a fasciated stem with disorganized meristem structures. We identified a homolog of TSK from tobacco BY-2 cells (NtTSK), which showed high sequence conservation both in the LGN domain and in leucine-rich repeats with AtTSK. The NtTSK gene was expressed during S phase of the cell cycle in tobacco BY-2 cells highly synchronized for cell division. The tsk mutants of Arabidopsis contained an increased proportion of cells with 4C nuclei and cells expressing cyclin B1 compared with the wild type. These results suggest that TSK is required during the cell cycle and defects of TSK cause the arrest of cell cycle progression at G2/M phase.

Original languageEnglish
Pages (from-to)736-742
Number of pages7
JournalPlant and Cell Physiology
Volume46
Issue number5
DOIs
Publication statusPublished - May 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

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