TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis

Takamasa Suzuki, Sakiko Nakajima, Soichi Inagaki, Makiko Hirano-Nakakita, Ken Matsuoka, Taku Demura, Hiroo Fukuda, Atsushi Morikami, Kenzo Nakamura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

TONSOKU(TSK)/MGOUN3/BRUSHY1 of Arabidopsis thaliana encodes a nuclear leucine-glycine-aspargine (LGN) domain protein implicated to be involved in genome maintenance, and mutants with defects in TSK show a fasciated stem with disorganized meristem structures. We identified a homolog of TSK from tobacco BY-2 cells (NtTSK), which showed high sequence conservation both in the LGN domain and in leucine-rich repeats with AtTSK. The NtTSK gene was expressed during S phase of the cell cycle in tobacco BY-2 cells highly synchronized for cell division. The tsk mutants of Arabidopsis contained an increased proportion of cells with 4C nuclei and cells expressing cyclin B1 compared with the wild type. These results suggest that TSK is required during the cell cycle and defects of TSK cause the arrest of cell cycle progression at G2/M phase.

Original languageEnglish
Pages (from-to)736-742
Number of pages7
JournalPlant and Cell Physiology
Volume46
Issue number5
DOIs
Publication statusPublished - May 1 2005

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S Phase
Arabidopsis
Leucine
interphase
cell cycle
Cell Cycle
leucine
Cell Division
Glycine
Tobacco
glycine (amino acid)
Cyclin B1
tobacco
Meristem
G2 Phase
cells
Cell Cycle Checkpoints
Cell Nucleus
mutants
cyclins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis. / Suzuki, Takamasa; Nakajima, Sakiko; Inagaki, Soichi; Hirano-Nakakita, Makiko; Matsuoka, Ken; Demura, Taku; Fukuda, Hiroo; Morikami, Atsushi; Nakamura, Kenzo.

In: Plant and Cell Physiology, Vol. 46, No. 5, 01.05.2005, p. 736-742.

Research output: Contribution to journalArticle

Suzuki, T, Nakajima, S, Inagaki, S, Hirano-Nakakita, M, Matsuoka, K, Demura, T, Fukuda, H, Morikami, A & Nakamura, K 2005, 'TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis', Plant and Cell Physiology, vol. 46, no. 5, pp. 736-742. https://doi.org/10.1093/pcp/pci082
Suzuki, Takamasa ; Nakajima, Sakiko ; Inagaki, Soichi ; Hirano-Nakakita, Makiko ; Matsuoka, Ken ; Demura, Taku ; Fukuda, Hiroo ; Morikami, Atsushi ; Nakamura, Kenzo. / TONSOKU is expressed in S phase of the cell cycle and its defect delays cell cycle progression in Arabidopsis. In: Plant and Cell Physiology. 2005 ; Vol. 46, No. 5. pp. 736-742.
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AU - Matsuoka, Ken

AU - Demura, Taku

AU - Fukuda, Hiroo

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