Tooth germ invagination from cell-cell interaction: Working hypothesis on mechanical instability

Hisako Imamura, Ritsuko Morita, Takafumi Iwaki, Takashi Tsuji, Kenichi Yoshikawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In the early stage of tooth germ development, the bud of the dental epithelium is invaginated by the underlying mesenchyme, resulting in the formation of a cap-like folded shape. This bud-to-cap transition plays a critical role in determining the steric design of the tooth. The epithelial-mesenchymal interaction within a tooth germ is essential for mediating the bud-to-cap transition. Here, we present a theoretical model to describe the autonomous process of the morphological transition, in which we introduce mechanical interactions among cells. Based on our observations, we assumed that peripheral cells of the dental epithelium bound tightly to each other to form an elastic sheet, and mesenchymal cells that covered the tooth germ would restrict its growth. By considering the time-dependent growth of cells, we were able to numerically show that the epithelium within the tooth germ buckled spontaneously, which is reminiscent of the cap-stage form. The difference in growth rates between the peripheral and interior parts of the dental epithelium, together with the steric size of the tooth germ, were determining factors for the number of invaginations. Our theoretical results provide a new hypothesis to explain the histological features of the tooth germ.

Original languageEnglish
Pages (from-to)284-291
Number of pages8
JournalJournal of Theoretical Biology
Volume382
DOIs
Publication statusPublished - Oct 7 2015

Fingerprint

Tooth Germ
Germ Cells
Cell Communication
teeth
Cell
Tooth
Epithelium
Interaction
Cells
cells
epithelium
Growth
Theoretical Model
buds
Interior
Mesoderm
Cap
Theoretical Models
cell growth

All Science Journal Classification (ASJC) codes

  • Statistics and Probability
  • Modelling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Applied Mathematics

Cite this

Tooth germ invagination from cell-cell interaction : Working hypothesis on mechanical instability. / Imamura, Hisako; Morita, Ritsuko; Iwaki, Takafumi; Tsuji, Takashi; Yoshikawa, Kenichi.

In: Journal of Theoretical Biology, Vol. 382, 07.10.2015, p. 284-291.

Research output: Contribution to journalArticle

Imamura, Hisako ; Morita, Ritsuko ; Iwaki, Takafumi ; Tsuji, Takashi ; Yoshikawa, Kenichi. / Tooth germ invagination from cell-cell interaction : Working hypothesis on mechanical instability. In: Journal of Theoretical Biology. 2015 ; Vol. 382. pp. 284-291.
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