TY - JOUR
T1 - Topographical control of neurite extension on stripe-patterned polymer films
AU - Tsuruma, Akinori
AU - Tanaka, Masaru
AU - Yamamoto, Sadaaki
AU - Fukushima, Nobuyuki
AU - Yabu, Hiroshi
AU - Shimomura, Masatsugu
N1 - Funding Information:
This study is supported by Grants-in-Aid from Japan Science and Technology Corporation (JST) and Special Coordination Funds for Promoting Science and Technology.
PY - 2006/8/15
Y1 - 2006/8/15
N2 - Controlling cell responses to material surfaces is important for tissue engineering. Topographical property on material surfaces can play a crucial role in directing nerve regeneration. We prepared regular stripe-patterned (groove-ridge pattern) polymer film by self-organization in order to control direction of neurite extension. Neural cells from cerebral cortex of embryonic day-14 mice were cultured on the film coated with poly-l-lysine. Here, we describe a complex and unusual contact guidance dependent on the pattern feature size. The neurites grew perpendicular to wide groove of 12.7 μm and wide ridge of 4.3 μm but parallel to narrow grooves (6.1 and 8.4 μm) and narrow ridge (2.2 and 3.6 μm). The neurites sprouted parallel to the narrow groove but uniformly on the wide groove. The emersion of neurites was suppressed and the length of neurites was longer compared with on a flat film. These results are of interest to understanding contact guidance and designing scaffold for neural network formation.
AB - Controlling cell responses to material surfaces is important for tissue engineering. Topographical property on material surfaces can play a crucial role in directing nerve regeneration. We prepared regular stripe-patterned (groove-ridge pattern) polymer film by self-organization in order to control direction of neurite extension. Neural cells from cerebral cortex of embryonic day-14 mice were cultured on the film coated with poly-l-lysine. Here, we describe a complex and unusual contact guidance dependent on the pattern feature size. The neurites grew perpendicular to wide groove of 12.7 μm and wide ridge of 4.3 μm but parallel to narrow grooves (6.1 and 8.4 μm) and narrow ridge (2.2 and 3.6 μm). The neurites sprouted parallel to the narrow groove but uniformly on the wide groove. The emersion of neurites was suppressed and the length of neurites was longer compared with on a flat film. These results are of interest to understanding contact guidance and designing scaffold for neural network formation.
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U2 - 10.1016/j.colsurfa.2005.11.100
DO - 10.1016/j.colsurfa.2005.11.100
M3 - Article
AN - SCOPUS:33746883877
SN - 0927-7757
VL - 284-285
SP - 470
EP - 474
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
ER -