Topography Influences Adherent Cell Regulation of Osteoclastogenesis

M. Nagasawa, L. F. Cooper, Y. Ogino, D. Mendonca, R. Liang, S. Yang, G. Mendonca, K. Uoshima

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18 Citations (Scopus)

Abstract

The importance of osteoclast-mediated bone resorption in the process of osseointegration has not been widely considered. In this study, cell culture was used to investigate the hypothesis that the function of implant-adherent bone marrow stromal cells (BMSCs) in osteoclastogenesis is influenced by surface topography. BMSCs isolated from femur and tibia of Sprague-Dawley rats were seeded onto 3 types of titanium surfaces (smooth, micro, and nano) and a control surface (tissue culture plastic) with or without osteogenic supplements. After 3 to 14 d, conditioned medium (CM) was collected. Subsequently, rat bone marrow-derived macrophages (BMMs) were cultured in media supplemented with soluble receptor activator of NF-ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) as well as BMSC CM from each of the 4 surfaces. Gene expression levels of soluble RANKL, osteoprotegerin, tumor necrosis factor α, and M-CSF in cultured BMSCs at different time points were measured by real-time polymerase chain reaction. The number of differentiated osteoclastic cells was determined after tartrate-resistant acid phosphatase staining. Analysis of variance and t test were used for statistical analysis. The expression of prominent osteoclast-promoting factors tumor necrosis factor α and M-CSF was increased by BMSCs cultured on both micro- and nanoscale titanium topographies (P < 0.01). BMSC CM contained a heat-labile factor that increased BMMs osteoclastogenesis. CM from both micro- and nanoscale surface-adherent BMSCs increased the osteoclast number (P < 0.01). Difference in surface topography altered BMSC phenotype and influenced BMM osteoclastogenesis. Local signaling by implant-adherent cells at the implant-bone interface may indirectly control osteoclastogenesis and bone accrual around endosseous implants.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalJournal of Dental Research
Volume95
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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    Nagasawa, M., Cooper, L. F., Ogino, Y., Mendonca, D., Liang, R., Yang, S., Mendonca, G., & Uoshima, K. (2016). Topography Influences Adherent Cell Regulation of Osteoclastogenesis. Journal of Dental Research, 95(3), 319-326. https://doi.org/10.1177/0022034515616760