Topography Influences Adherent Cell Regulation of Osteoclastogenesis

M. Nagasawa, L. F. Cooper, Yoichiro Ogino, D. Mendonca, R. Liang, S. Yang, G. Mendonca, K. Uoshima

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The importance of osteoclast-mediated bone resorption in the process of osseointegration has not been widely considered. In this study, cell culture was used to investigate the hypothesis that the function of implant-adherent bone marrow stromal cells (BMSCs) in osteoclastogenesis is influenced by surface topography. BMSCs isolated from femur and tibia of Sprague-Dawley rats were seeded onto 3 types of titanium surfaces (smooth, micro, and nano) and a control surface (tissue culture plastic) with or without osteogenic supplements. After 3 to 14 d, conditioned medium (CM) was collected. Subsequently, rat bone marrow-derived macrophages (BMMs) were cultured in media supplemented with soluble receptor activator of NF-ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) as well as BMSC CM from each of the 4 surfaces. Gene expression levels of soluble RANKL, osteoprotegerin, tumor necrosis factor α, and M-CSF in cultured BMSCs at different time points were measured by real-time polymerase chain reaction. The number of differentiated osteoclastic cells was determined after tartrate-resistant acid phosphatase staining. Analysis of variance and t test were used for statistical analysis. The expression of prominent osteoclast-promoting factors tumor necrosis factor α and M-CSF was increased by BMSCs cultured on both micro- and nanoscale titanium topographies (P < 0.01). BMSC CM contained a heat-labile factor that increased BMMs osteoclastogenesis. CM from both micro- and nanoscale surface-adherent BMSCs increased the osteoclast number (P < 0.01). Difference in surface topography altered BMSC phenotype and influenced BMM osteoclastogenesis. Local signaling by implant-adherent cells at the implant-bone interface may indirectly control osteoclastogenesis and bone accrual around endosseous implants.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalJournal of Dental Research
Volume95
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

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Mesenchymal Stromal Cells
Osteogenesis
Conditioned Culture Medium
Macrophage Colony-Stimulating Factor
Osteoclasts
Macrophages
Titanium
Tumor Necrosis Factor-alpha
Osseointegration
Osteoprotegerin
Bone Resorption
Tibia
Femur
Plastics
Sprague Dawley Rats
Real-Time Polymerase Chain Reaction
Cultured Cells
Analysis of Variance
Cell Culture Techniques
Hot Temperature

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Nagasawa, M., Cooper, L. F., Ogino, Y., Mendonca, D., Liang, R., Yang, S., ... Uoshima, K. (2016). Topography Influences Adherent Cell Regulation of Osteoclastogenesis. Journal of Dental Research, 95(3), 319-326. https://doi.org/10.1177/0022034515616760

Topography Influences Adherent Cell Regulation of Osteoclastogenesis. / Nagasawa, M.; Cooper, L. F.; Ogino, Yoichiro; Mendonca, D.; Liang, R.; Yang, S.; Mendonca, G.; Uoshima, K.

In: Journal of Dental Research, Vol. 95, No. 3, 01.03.2016, p. 319-326.

Research output: Contribution to journalArticle

Nagasawa, M, Cooper, LF, Ogino, Y, Mendonca, D, Liang, R, Yang, S, Mendonca, G & Uoshima, K 2016, 'Topography Influences Adherent Cell Regulation of Osteoclastogenesis', Journal of Dental Research, vol. 95, no. 3, pp. 319-326. https://doi.org/10.1177/0022034515616760
Nagasawa, M. ; Cooper, L. F. ; Ogino, Yoichiro ; Mendonca, D. ; Liang, R. ; Yang, S. ; Mendonca, G. ; Uoshima, K. / Topography Influences Adherent Cell Regulation of Osteoclastogenesis. In: Journal of Dental Research. 2016 ; Vol. 95, No. 3. pp. 319-326.
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