Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast

Masamune Aihara, Xiulian Jin, Yusuke Kurihara, Yutaka Yoshida, Yuichi Matsushima, Masahide Oku, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Tadashi Yamamoto, Yasuyoshi Sakai, Dongchon Kang, Tomotake Kanki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

When mitophagy is induced in Saccharomyces cerevisiae, the mitochondrial outer membrane protein ScAtg32 interacts with the cytosolic adaptor protein ScAtg11. ScAtg11 then delivers the mitochondria to the pre-autophagosomal structure for autophagic degradation. Despite the importance of ScAtg32 for mitophagy, the expression and functional regulation of ScAtg32 are poorly understood. In this study, we identified and characterized the ScAtg32 homolog in Pichia pastoris (PpAtg32). Interestingly, we found that PpAtg32 was barely expressed before induction of mitophagy and was rapidly expressed after induction of mitophagy by starvation. Additionally, PpAtg32 was phosphorylated when mitophagy was induced. We found that PpAtg32 expression was suppressed by Tor and the downstream PpSin3-PpRpd3 complex. Inhibition of Tor by rapamycin induced PpAtg32 expression, but could neither phosphorylate PpAtg32 nor induce mitophagy. Based on these findings, we conclude that the Tor and PpSin3-PpRpd3 pathway regulates PpAtg32 expression, but not PpAtg32 phosphorylation.

Original languageEnglish
Pages (from-to)3184-3196
Number of pages13
JournalJournal of Cell Science
Volume127
Issue number14
DOIs
Publication statusPublished - Jan 1 2014

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Mitochondrial Degradation
Yeasts
Proteins
Pichia
Sirolimus
Starvation
Saccharomyces cerevisiae
Mitochondria
Membrane Proteins
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast. / Aihara, Masamune; Jin, Xiulian; Kurihara, Yusuke; Yoshida, Yutaka; Matsushima, Yuichi; Oku, Masahide; Hirota, Yuko; Saigusa, Tetsu; Aoki, Yoshimasa; Uchiumi, Takeshi; Yamamoto, Tadashi; Sakai, Yasuyoshi; Kang, Dongchon; Kanki, Tomotake.

In: Journal of Cell Science, Vol. 127, No. 14, 01.01.2014, p. 3184-3196.

Research output: Contribution to journalArticle

Aihara, M, Jin, X, Kurihara, Y, Yoshida, Y, Matsushima, Y, Oku, M, Hirota, Y, Saigusa, T, Aoki, Y, Uchiumi, T, Yamamoto, T, Sakai, Y, Kang, D & Kanki, T 2014, 'Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast', Journal of Cell Science, vol. 127, no. 14, pp. 3184-3196. https://doi.org/10.1242/jcs.153254
Aihara, Masamune ; Jin, Xiulian ; Kurihara, Yusuke ; Yoshida, Yutaka ; Matsushima, Yuichi ; Oku, Masahide ; Hirota, Yuko ; Saigusa, Tetsu ; Aoki, Yoshimasa ; Uchiumi, Takeshi ; Yamamoto, Tadashi ; Sakai, Yasuyoshi ; Kang, Dongchon ; Kanki, Tomotake. / Tor and the Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32 in yeast. In: Journal of Cell Science. 2014 ; Vol. 127, No. 14. pp. 3184-3196.
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