Total fractionation of green tea residue by microwave-assisted alkaline pretreatment and enzymatic hydrolysis

Shuntaro Tsubaki, Jun ichi Azuma

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25 Citations (Scopus)

Abstract

Total refinery of constituents of green tea residue was achieved by combination of microwave-assisted alkaline pretreatment and enzymatic hydrolysis. Alkaline pretreatment was effective at separating pectic polysaccharides, protein, phenolic compounds and aliphatic compounds (probably originating from cuticular components), and the solubilization rate was attained 64-74% by heating at 120-200 °C. The higher heating value (HHV) of alkali-soluble fraction attained 20.1. MJ/kg, indicating its usability as black-liquor-like biofuel. Successive cellulolytic enzymatic hydrolysis mainly converted cellulose into glucose and attained the maximum solubilization rate of 89%. Final residue was predominantly composed of aliphatic cuticular components with high proportion in 9,10,18-trihydroxyoctadecanoic acid (30.1-48.6%). These cuticular components are potential alternative feedstock for aliphatic compounds commonly found in oil plants.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalBioresource Technology
Volume131
DOIs
Publication statusPublished - Mar 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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