Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization

H Kondo, M Miura, I Nakagaki, S Sasaki, Y Itokawa

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The movements of trace elements and the level of oxidative stress in the soleus, a typical slow red muscle which, atrophied by immobilization, were investigated in designated intervals. Male Wistar rats (14 wk old) whose one ankle joints were immobilized in the extended position were killed after 4, 8, and 12 days. Fe, Zn, Mn, and Cu concentrations and the levels of thiobarbituric acid-reactive substance (TBARS) and glutathione were measured. The rate of atrophy increased rapidly until the 8th day and slowly after that. In whole muscle, Fe concentration kept increasing, and Zn and Mn increased temporarily. Their subcellular distributions also changed; especially, the Fe level of the microsomal fraction kept increasing and reached threefold at 12 days. Increased TBARS and glutathione disulfide and decreased total glutathione indicated the increased oxidative stress in atrophy, which might result from an increased Fe level, especially that of the microsomal fraction. Vitamin E injection lessened the rate of atrophy, which showed that oxidative stress accelerated muscle atrophy. This might be mediated by increased intracellular Ca. Also metallothionein was induced in muscle atrophy.

Original languageEnglish
Pages (from-to)E583-90
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number5 Pt 1
Publication statusPublished - May 1992

Fingerprint

Trace Elements
Immobilization
Atrophy
Skeletal Muscle
Oxidative Stress
Muscular Atrophy
Thiobarbituric Acid Reactive Substances
Glutathione
Muscles
Ankle Joint
Glutathione Disulfide
Metallothionein
Vitamin E
Wistar Rats
Injections

Cite this

Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization. / Kondo, H; Miura, M; Nakagaki, I; Sasaki, S; Itokawa, Y.

In: American Journal of Physiology - Cell Physiology, Vol. 262, No. 5 Pt 1, 05.1992, p. E583-90.

Research output: Contribution to journalArticle

Kondo, H ; Miura, M ; Nakagaki, I ; Sasaki, S ; Itokawa, Y. / Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization. In: American Journal of Physiology - Cell Physiology. 1992 ; Vol. 262, No. 5 Pt 1. pp. E583-90.
@article{ab77f90f8c8e418abf8cafd705c35ab9,
title = "Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization",
abstract = "The movements of trace elements and the level of oxidative stress in the soleus, a typical slow red muscle which, atrophied by immobilization, were investigated in designated intervals. Male Wistar rats (14 wk old) whose one ankle joints were immobilized in the extended position were killed after 4, 8, and 12 days. Fe, Zn, Mn, and Cu concentrations and the levels of thiobarbituric acid-reactive substance (TBARS) and glutathione were measured. The rate of atrophy increased rapidly until the 8th day and slowly after that. In whole muscle, Fe concentration kept increasing, and Zn and Mn increased temporarily. Their subcellular distributions also changed; especially, the Fe level of the microsomal fraction kept increasing and reached threefold at 12 days. Increased TBARS and glutathione disulfide and decreased total glutathione indicated the increased oxidative stress in atrophy, which might result from an increased Fe level, especially that of the microsomal fraction. Vitamin E injection lessened the rate of atrophy, which showed that oxidative stress accelerated muscle atrophy. This might be mediated by increased intracellular Ca. Also metallothionein was induced in muscle atrophy.",
author = "H Kondo and M Miura and I Nakagaki and S Sasaki and Y Itokawa",
year = "1992",
month = "5",
language = "English",
volume = "262",
pages = "E583--90",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "5 Pt 1",

}

TY - JOUR

T1 - Trace element movement and oxidative stress in skeletal muscle atrophied by immobilization

AU - Kondo, H

AU - Miura, M

AU - Nakagaki, I

AU - Sasaki, S

AU - Itokawa, Y

PY - 1992/5

Y1 - 1992/5

N2 - The movements of trace elements and the level of oxidative stress in the soleus, a typical slow red muscle which, atrophied by immobilization, were investigated in designated intervals. Male Wistar rats (14 wk old) whose one ankle joints were immobilized in the extended position were killed after 4, 8, and 12 days. Fe, Zn, Mn, and Cu concentrations and the levels of thiobarbituric acid-reactive substance (TBARS) and glutathione were measured. The rate of atrophy increased rapidly until the 8th day and slowly after that. In whole muscle, Fe concentration kept increasing, and Zn and Mn increased temporarily. Their subcellular distributions also changed; especially, the Fe level of the microsomal fraction kept increasing and reached threefold at 12 days. Increased TBARS and glutathione disulfide and decreased total glutathione indicated the increased oxidative stress in atrophy, which might result from an increased Fe level, especially that of the microsomal fraction. Vitamin E injection lessened the rate of atrophy, which showed that oxidative stress accelerated muscle atrophy. This might be mediated by increased intracellular Ca. Also metallothionein was induced in muscle atrophy.

AB - The movements of trace elements and the level of oxidative stress in the soleus, a typical slow red muscle which, atrophied by immobilization, were investigated in designated intervals. Male Wistar rats (14 wk old) whose one ankle joints were immobilized in the extended position were killed after 4, 8, and 12 days. Fe, Zn, Mn, and Cu concentrations and the levels of thiobarbituric acid-reactive substance (TBARS) and glutathione were measured. The rate of atrophy increased rapidly until the 8th day and slowly after that. In whole muscle, Fe concentration kept increasing, and Zn and Mn increased temporarily. Their subcellular distributions also changed; especially, the Fe level of the microsomal fraction kept increasing and reached threefold at 12 days. Increased TBARS and glutathione disulfide and decreased total glutathione indicated the increased oxidative stress in atrophy, which might result from an increased Fe level, especially that of the microsomal fraction. Vitamin E injection lessened the rate of atrophy, which showed that oxidative stress accelerated muscle atrophy. This might be mediated by increased intracellular Ca. Also metallothionein was induced in muscle atrophy.

M3 - Article

C2 - 1590370

VL - 262

SP - E583-90

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 5 Pt 1

ER -