Abstract
Here we demonstrate that a molecular motion detector in microfluidic channel can track nucleolytic and proteolytic degradations at a single molecule level. Central to these results is our observation that the microfluidic control worked well to visualize transient molecular processes clearly. A geometric analysis of the time course of molecular trajectories indicated hydrolysis rates and induction periods of each reaction.
| Original language | English |
|---|---|
| Title of host publication | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
| Pages | 1439-1441 |
| Number of pages | 3 |
| Publication status | Published - Dec 1 2010 |
| Externally published | Yes |
| Event | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: Oct 3 2010 → Oct 7 2010 |
Publication series
| Name | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Volume | 3 |
Other
| Other | 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 |
|---|---|
| Country | Netherlands |
| City | Groningen |
| Period | 10/3/10 → 10/7/10 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
Onoshima, D., Kaji, N., Tokeshi, M., & Baba, Y. (2010). Tracking of single DNA and protein molecules undergoing enzymatic degradation in fluid. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 1439-1441). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 3).