TY - GEN
T1 - Tracking of single DNA and protein molecules undergoing enzymatic degradation in fluid
AU - Onoshima, D.
AU - Kaji, N.
AU - Tokeshi, M.
AU - Baba, Y.
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Here we demonstrate that a molecular motion detector in microfluidic channel can track nucleolytic and proteolytic degradations at a single molecule level. Central to these results is our observation that the microfluidic control worked well to visualize transient molecular processes clearly. A geometric analysis of the time course of molecular trajectories indicated hydrolysis rates and induction periods of each reaction.
AB - Here we demonstrate that a molecular motion detector in microfluidic channel can track nucleolytic and proteolytic degradations at a single molecule level. Central to these results is our observation that the microfluidic control worked well to visualize transient molecular processes clearly. A geometric analysis of the time course of molecular trajectories indicated hydrolysis rates and induction periods of each reaction.
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M3 - Conference contribution
AN - SCOPUS:84884305816
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1439
EP - 1441
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -