Tracking of single DNA and protein molecules undergoing enzymatic degradation in fluid

D. Onoshima, N. Kaji, M. Tokeshi, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we demonstrate that a molecular motion detector in microfluidic channel can track nucleolytic and proteolytic degradations at a single molecule level. Central to these results is our observation that the microfluidic control worked well to visualize transient molecular processes clearly. A geometric analysis of the time course of molecular trajectories indicated hydrolysis rates and induction periods of each reaction.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1439-1441
Number of pages3
Publication statusPublished - Dec 1 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

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All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Onoshima, D., Kaji, N., Tokeshi, M., & Baba, Y. (2010). Tracking of single DNA and protein molecules undergoing enzymatic degradation in fluid. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 1439-1441). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 3).