Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures

Yuri Maeda, Hiroyuki Furuta, Yoshiya Ikawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number3
DOIs
Publication statusPublished - Mar 1 2011

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Molecular Probes
RNA
Monitoring
Structural dynamics
Chemical modification

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures. / Maeda, Yuri; Furuta, Hiroyuki; Ikawa, Yoshiya.

In: Journal of Bioscience and Bioengineering, Vol. 111, No. 3, 01.03.2011, p. 370-376.

Research output: Contribution to journalArticle

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