Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures

Yuri Maeda, Hiroyuki Furuta, Yoshiya Ikawa

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Abstract

As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number3
DOIs
Publication statusPublished - Mar 1 2011

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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