TY - JOUR
T1 - Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures
AU - Maeda, Yuri
AU - Furuta, Hiroyuki
AU - Ikawa, Yoshiya
PY - 2011/3/1
Y1 - 2011/3/1
N2 - As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.
AB - As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.
UR - http://www.scopus.com/inward/record.url?scp=79951811667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951811667&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2010.11.007
DO - 10.1016/j.jbiosc.2010.11.007
M3 - Article
C2 - 21163698
AN - SCOPUS:79951811667
VL - 111
SP - 370
EP - 376
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -