Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures

Yuri Maeda, Hiroyuki Furuta, Yoshiya Ikawa

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)

    Abstract

    As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.

    Original languageEnglish
    Pages (from-to)370-376
    Number of pages7
    JournalJournal of Bioscience and Bioengineering
    Volume111
    Issue number3
    DOIs
    Publication statusPublished - Mar 2011

    All Science Journal Classification (ASJC) codes

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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