Transcript profiling in plastid arginine tRNA-CCG gene knockout moss: Construction of Physcomitrella patens plastid DNA microarray

Takahiro Nakamura, C. Sugiura, Y. Kobayashi, M. Sugita

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The moss Physcomitrella patens is a newly established model plant that is widely used for the characterization of gene function by targeted gene knockout or over-expression. The target gene disruption occurs in both the nuclear and chloroplast genomes. We applied DNA microarray technology to the P. patens plastid genome for large-scale analysis of transcripts. A microarray was constructed containing 108 DNA fragments to detect all annotated plastid genes. We analyzed the transcript profile in a knockout transformant for the arginine tRNA gene, trnR-CCG, and confirmed previous results that rbcL and psal transcripts accumulate in similar levels to wild-type moss, and accD transcript level is higher than those of wild-type moss. Additionally, the plastid DNA microarray revealed that most plastid genes were expressed at similar levels in wild-type and transformant mosses. This indicates that tmR-CCG is not essential for the expression of plastid genes.

Original languageEnglish
Pages (from-to)258-265
Number of pages8
JournalPlant Biology
Volume7
Issue number3
DOIs
Publication statusPublished - May 1 2005

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Physcomitrella patens
plastid DNA
plastid
gene targeting
moss
plastids
arginine
mosses and liverworts
DNA
gene
genes
microarray technology
genome
nuclear genome
DNA microarrays
chloroplast

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cite this

Transcript profiling in plastid arginine tRNA-CCG gene knockout moss : Construction of Physcomitrella patens plastid DNA microarray. / Nakamura, Takahiro; Sugiura, C.; Kobayashi, Y.; Sugita, M.

In: Plant Biology, Vol. 7, No. 3, 01.05.2005, p. 258-265.

Research output: Contribution to journalArticle

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