TY - JOUR
T1 - Transcript profiling in plastid arginine tRNA-CCG gene knockout moss
T2 - Construction of Physcomitrella patens plastid DNA microarray
AU - Nakamura, T.
AU - Sugiura, C.
AU - Kobayashi, Y.
AU - Sugita, M.
PY - 2005/5/1
Y1 - 2005/5/1
N2 - The moss Physcomitrella patens is a newly established model plant that is widely used for the characterization of gene function by targeted gene knockout or over-expression. The target gene disruption occurs in both the nuclear and chloroplast genomes. We applied DNA microarray technology to the P. patens plastid genome for large-scale analysis of transcripts. A microarray was constructed containing 108 DNA fragments to detect all annotated plastid genes. We analyzed the transcript profile in a knockout transformant for the arginine tRNA gene, trnR-CCG, and confirmed previous results that rbcL and psal transcripts accumulate in similar levels to wild-type moss, and accD transcript level is higher than those of wild-type moss. Additionally, the plastid DNA microarray revealed that most plastid genes were expressed at similar levels in wild-type and transformant mosses. This indicates that tmR-CCG is not essential for the expression of plastid genes.
AB - The moss Physcomitrella patens is a newly established model plant that is widely used for the characterization of gene function by targeted gene knockout or over-expression. The target gene disruption occurs in both the nuclear and chloroplast genomes. We applied DNA microarray technology to the P. patens plastid genome for large-scale analysis of transcripts. A microarray was constructed containing 108 DNA fragments to detect all annotated plastid genes. We analyzed the transcript profile in a knockout transformant for the arginine tRNA gene, trnR-CCG, and confirmed previous results that rbcL and psal transcripts accumulate in similar levels to wild-type moss, and accD transcript level is higher than those of wild-type moss. Additionally, the plastid DNA microarray revealed that most plastid genes were expressed at similar levels in wild-type and transformant mosses. This indicates that tmR-CCG is not essential for the expression of plastid genes.
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U2 - 10.1055/s-2005-865620
DO - 10.1055/s-2005-865620
M3 - Article
C2 - 15912445
AN - SCOPUS:20144384255
SN - 1435-8603
VL - 7
SP - 258
EP - 265
JO - Berichte der Deutschen Botanischen Gesellschaft
JF - Berichte der Deutschen Botanischen Gesellschaft
IS - 3
ER -