Transcription factor IRF1 is responsible for IRF8-mediated IL-1β expression in reactive microglia

Takahiro Masuda, Shosuke Iwamoto, Satsuki Mikuriya, Hidetoshi Saitoh, Tomohiko Tamura, Tsuda Makoto, Kazuhide Inoue

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Interferon regulatory factor-8 (IRF8) plays a crucial role in the transformation of microglia to a reactive state by regulating the expression of various genes. In the present study, we show that IRF1 is required for IRF8-induced gene expression in microglia. Peripheral nerve injury induced IRF1 gene upregulation in the spinal microglia in an IRF8-dependent manner. IRF8 transduction in cultured microglia induced de novo gene expression of IRF1. Importantly, knockdown of the IRF1 gene in IRF8-transduced microglia prevented upregulation of interleukin-1β (IL-1β). Therefore, our findings suggest that expression of IL-1β is dependent on IRF1 in IRF8-expressing reactive microglia.

Original languageEnglish
Pages (from-to)216-220
Number of pages5
JournalJournal of Pharmacological Sciences
Volume128
Issue number4
DOIs
Publication statusPublished - Sep 4 2015

Fingerprint

Microglia
Interleukin-1
Transcription Factors
Gene Expression
Up-Regulation
Gene Knockdown Techniques
Peripheral Nerve Injuries
interferon regulatory factor-8
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

Transcription factor IRF1 is responsible for IRF8-mediated IL-1β expression in reactive microglia. / Masuda, Takahiro; Iwamoto, Shosuke; Mikuriya, Satsuki; Saitoh, Hidetoshi; Tamura, Tomohiko; Makoto, Tsuda; Inoue, Kazuhide.

In: Journal of Pharmacological Sciences, Vol. 128, No. 4, 04.09.2015, p. 216-220.

Research output: Contribution to journalArticle

@article{b38a21d3d11a47a18bb7c3d94d879e20,
title = "Transcription factor IRF1 is responsible for IRF8-mediated IL-1β expression in reactive microglia",
abstract = "Interferon regulatory factor-8 (IRF8) plays a crucial role in the transformation of microglia to a reactive state by regulating the expression of various genes. In the present study, we show that IRF1 is required for IRF8-induced gene expression in microglia. Peripheral nerve injury induced IRF1 gene upregulation in the spinal microglia in an IRF8-dependent manner. IRF8 transduction in cultured microglia induced de novo gene expression of IRF1. Importantly, knockdown of the IRF1 gene in IRF8-transduced microglia prevented upregulation of interleukin-1β (IL-1β). Therefore, our findings suggest that expression of IL-1β is dependent on IRF1 in IRF8-expressing reactive microglia.",
author = "Takahiro Masuda and Shosuke Iwamoto and Satsuki Mikuriya and Hidetoshi Saitoh and Tomohiko Tamura and Tsuda Makoto and Kazuhide Inoue",
year = "2015",
month = "9",
day = "4",
doi = "10.1016/j.jphs.2015.08.002",
language = "English",
volume = "128",
pages = "216--220",
journal = "Journal of Pharmacological Sciences",
issn = "1347-8613",
publisher = "Japanese Pharmacological Society",
number = "4",

}

TY - JOUR

T1 - Transcription factor IRF1 is responsible for IRF8-mediated IL-1β expression in reactive microglia

AU - Masuda, Takahiro

AU - Iwamoto, Shosuke

AU - Mikuriya, Satsuki

AU - Saitoh, Hidetoshi

AU - Tamura, Tomohiko

AU - Makoto, Tsuda

AU - Inoue, Kazuhide

PY - 2015/9/4

Y1 - 2015/9/4

N2 - Interferon regulatory factor-8 (IRF8) plays a crucial role in the transformation of microglia to a reactive state by regulating the expression of various genes. In the present study, we show that IRF1 is required for IRF8-induced gene expression in microglia. Peripheral nerve injury induced IRF1 gene upregulation in the spinal microglia in an IRF8-dependent manner. IRF8 transduction in cultured microglia induced de novo gene expression of IRF1. Importantly, knockdown of the IRF1 gene in IRF8-transduced microglia prevented upregulation of interleukin-1β (IL-1β). Therefore, our findings suggest that expression of IL-1β is dependent on IRF1 in IRF8-expressing reactive microglia.

AB - Interferon regulatory factor-8 (IRF8) plays a crucial role in the transformation of microglia to a reactive state by regulating the expression of various genes. In the present study, we show that IRF1 is required for IRF8-induced gene expression in microglia. Peripheral nerve injury induced IRF1 gene upregulation in the spinal microglia in an IRF8-dependent manner. IRF8 transduction in cultured microglia induced de novo gene expression of IRF1. Importantly, knockdown of the IRF1 gene in IRF8-transduced microglia prevented upregulation of interleukin-1β (IL-1β). Therefore, our findings suggest that expression of IL-1β is dependent on IRF1 in IRF8-expressing reactive microglia.

UR - http://www.scopus.com/inward/record.url?scp=84940790549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940790549&partnerID=8YFLogxK

U2 - 10.1016/j.jphs.2015.08.002

DO - 10.1016/j.jphs.2015.08.002

M3 - Article

C2 - 26318672

AN - SCOPUS:84940790549

VL - 128

SP - 216

EP - 220

JO - Journal of Pharmacological Sciences

JF - Journal of Pharmacological Sciences

SN - 1347-8613

IS - 4

ER -