Abstract
Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.
| Original language | English |
|---|---|
| Pages (from-to) | 344-349 |
| Number of pages | 6 |
| Journal | Marine Pollution Bulletin |
| Volume | 109 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 15 2016 |
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All Science Journal Classification (ASJC) codes
- Oceanography
- Aquatic Science
- Pollution
Cite this
Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.). / Ito, Mana; Ito, Katsutoshi; Ohta, Kohei; Hano, Takeshi; Onduka, Toshimitsu; Mochida, Kazuhiko.
In: Marine Pollution Bulletin, Vol. 109, No. 1, 15.08.2016, p. 344-349.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)
AU - Ito, Mana
AU - Ito, Katsutoshi
AU - Ohta, Kohei
AU - Hano, Takeshi
AU - Onduka, Toshimitsu
AU - Mochida, Kazuhiko
PY - 2016/8/15
Y1 - 2016/8/15
N2 - Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.
AB - Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.
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UR - http://www.scopus.com/inward/citedby.url?scp=84970021388&partnerID=8YFLogxK
U2 - 10.1016/j.marpolbul.2016.05.055
DO - 10.1016/j.marpolbul.2016.05.055
M3 - Article
C2 - 27251443
AN - SCOPUS:84970021388
VL - 109
SP - 344
EP - 349
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
SN - 0025-326X
IS - 1
ER -