Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)

Mana Ito, Katsutoshi Ito, Kohei Ohta, Takeshi Hano, Toshimitsu Onduka, Kazuhiko Mochida

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.

Original languageEnglish
Pages (from-to)344-349
Number of pages6
JournalMarine Pollution Bulletin
Volume109
Issue number1
DOIs
Publication statusPublished - Aug 15 2016

Fingerprint

body regions
Oligochaeta
Transcription
exposure duration
Sediments
transcription (genetics)
Genes
pollutants
polycyclic aromatic hydrocarbons
cytochrome P-450
sediments
gene
Polycyclic aromatic hydrocarbons
cytochrome
sediment contamination
PAH
temperature
genes
marine sediments
sediment pollution

All Science Journal Classification (ASJC) codes

  • Oceanography
  • Aquatic Science
  • Pollution

Cite this

Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.). / Ito, Mana; Ito, Katsutoshi; Ohta, Kohei; Hano, Takeshi; Onduka, Toshimitsu; Mochida, Kazuhiko.

In: Marine Pollution Bulletin, Vol. 109, No. 1, 15.08.2016, p. 344-349.

Research output: Contribution to journalArticle

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AU - Hano, Takeshi

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