TY - JOUR
T1 - Transcription of a novel P450 gene varies with some factors (pollutant exposure, temperature, time, and body region) in a marine oligochaete (Thalassodrilides sp.)
AU - Ito, Mana
AU - Ito, Katsutoshi
AU - Ohta, Kohei
AU - Hano, Takeshi
AU - Onduka, Toshimitsu
AU - Mochida, Kazuhiko
PY - 2016/8/15
Y1 - 2016/8/15
N2 - Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.
AB - Cytochrome P450 (CYP) enzymes play important roles in the metabolism of exogenous compounds such as polycyclic aromatic hydrocarbons (PAHs). A novel, full-length CYP gene (CYP4V30) was identified in the oligochaete Thalassodrilides sp. CYP4V30 mRNA expression was studied in worms exposed to PAH-polluted (Σ16PAHs; 37441 ng/g dry weight) or unpolluted (Σ16PAHs; 19 ng/g dry weight) sediment. CYP4V30 expression was much higher in worms exposed to contaminated sediments than in those exposed to unpolluted sediments at some temperatures (20 and 25 °C) and exposure durations (11-fold increase at 20 °C, 10-day exposure), but not at 15 °C or other exposure durations (P < 0.05). CYP4V30 mRNA expression was higher in the middle of the body than in the posterior (P < 0.05). The variation in transcriptional response with exposure time, temperature, and body region indicates that these factors should be considered when monitoring marine sediment pollution.
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U2 - 10.1016/j.marpolbul.2016.05.055
DO - 10.1016/j.marpolbul.2016.05.055
M3 - Article
C2 - 27251443
AN - SCOPUS:84970021388
VL - 109
SP - 344
EP - 349
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
SN - 0025-326X
IS - 1
ER -