Abstract
The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.
| Original language | English |
|---|---|
| Pages (from-to) | 171-176 |
| Number of pages | 6 |
| Journal | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Volume | 1759 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - Mar 1 2006 |
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All Science Journal Classification (ASJC) codes
- Structural Biology
- Biophysics
- Biochemistry
- Genetics
Cite this
Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines. / Fukuzawa, Hideaki; Kiyoshima, Tamotsu; Kobayashi, Ieyoshi; Ozeki, Satoru; Sakai, Hidetaka.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1759, No. 3-4, 01.03.2006, p. 171-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines
AU - Fukuzawa, Hideaki
AU - Kiyoshima, Tamotsu
AU - Kobayashi, Ieyoshi
AU - Ozeki, Satoru
AU - Sakai, Hidetaka
PY - 2006/3/1
Y1 - 2006/3/1
N2 - The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.
AB - The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.
UR - http://www.scopus.com/inward/record.url?scp=33744546499&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744546499&partnerID=8YFLogxK
U2 - 10.1016/j.bbaexp.2006.03.004
DO - 10.1016/j.bbaexp.2006.03.004
M3 - Article
C2 - 16675044
AN - SCOPUS:33744546499
VL - 1759
SP - 171
EP - 176
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3-4
ER -