Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines

Hideaki Fukuzawa, Tamotsu Kiyoshima, Ieyoshi Kobayashi, Satoru Ozeki, Hidetaka Sakai

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.

Original languageEnglish
Pages (from-to)171-176
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1759
Issue number3-4
DOIs
Publication statusPublished - Mar 1 2006

Fingerprint

Transcription
Human Activities
Squamous Cell Carcinoma
Genes
Cells
Cell Line
Assays
Electrophoretic mobility
5' Flanking Region
Nucleic Acid Regulatory Sequences
Electrophoretic Mobility Shift Assay
Epithelial Cells
Psoriasis
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines. / Fukuzawa, Hideaki; Kiyoshima, Tamotsu; Kobayashi, Ieyoshi; Ozeki, Satoru; Sakai, Hidetaka.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1759, No. 3-4, 01.03.2006, p. 171-176.

Research output: Contribution to journalArticle

Fukuzawa, Hideaki ; Kiyoshima, Tamotsu ; Kobayashi, Ieyoshi ; Ozeki, Satoru ; Sakai, Hidetaka. / Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 2006 ; Vol. 1759, No. 3-4. pp. 171-176.
@article{c8a02d5da36a414fbd5b0defbf7c00a3,
title = "Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines",
abstract = "The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.",
author = "Hideaki Fukuzawa and Tamotsu Kiyoshima and Ieyoshi Kobayashi and Satoru Ozeki and Hidetaka Sakai",
year = "2006",
month = "3",
day = "1",
doi = "10.1016/j.bbaexp.2006.03.004",
language = "English",
volume = "1759",
pages = "171--176",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3-4",

}

TY - JOUR

T1 - Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines

AU - Fukuzawa, Hideaki

AU - Kiyoshima, Tamotsu

AU - Kobayashi, Ieyoshi

AU - Ozeki, Satoru

AU - Sakai, Hidetaka

PY - 2006/3/1

Y1 - 2006/3/1

N2 - The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.

AB - The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.

UR - http://www.scopus.com/inward/record.url?scp=33744546499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744546499&partnerID=8YFLogxK

U2 - 10.1016/j.bbaexp.2006.03.004

DO - 10.1016/j.bbaexp.2006.03.004

M3 - Article

C2 - 16675044

AN - SCOPUS:33744546499

VL - 1759

SP - 171

EP - 176

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3-4

ER -