TY - JOUR
T1 - Transcription promoter activity of the human S100A7 gene in oral squamous cell carcinoma cell lines
AU - Fukuzawa, Hideaki
AU - Kiyoshima, Tamotsu
AU - Kobayashi, Ieyoshi
AU - Ozeki, Satoru
AU - Sakai, Hidetaka
N1 - Funding Information:
This work was supported in part by Grants-in-aid for Frontier Research to S.O., and for Scientific Research in a Priority Area from the Ministry of Education, Culture, Sports, Science and Technology of Japan (17390548 and 17659643 to S.O. and 15591937 to T.K.).
PY - 2006/3
Y1 - 2006/3
N2 - The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.
AB - The S100A7 (psoriasin) gene has been shown to be markedly over-expressed in squamous cell carcinomas (SCCs) as well as in psoriasis. We herein examined the S100A7 gene promoter activity in human oral SCC cell lines to identify the putative SCC-specific regulatory regions for the S100A7 transcription. Functional deletion assays of 5′-flanking region demonstrated that the segments, (-1513 to -988), (-1954 to -1513) and (-3040 to -2578), play important roles in the transcription activity in the oral SCCs. The internal deletion of the short segments, (-1248 to -1110), (-1109 to -988) and (-1248 to -988), decreased this activity. These segments cloned upstream of the heterologous promoter increased the promoter activity in oral SCC cell line. Electrophoretic mobility shift assays, using the sequence segmental probes, (-1248 to -1110) and (-1109 to -988), showed different DNA-protein complex patterns depending on the types of used cell lines. One of the complexes was only observed in the oral SCCs. These data suggested that the segment from -1513 to -988 contains up-regulatory elements for the transcription activity of the S100A7 gene in oral SCCs.
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U2 - 10.1016/j.bbaexp.2006.03.004
DO - 10.1016/j.bbaexp.2006.03.004
M3 - Article
C2 - 16675044
AN - SCOPUS:33744546499
VL - 1759
SP - 171
EP - 176
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3-4
ER -