Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis

Koichi Akashi, Xi He, Jie Chen, Hiromi Iwasaki, Chao Niu, Brooke Steenhard, Jiwang Zhang, Jeff Haug, Linheng Li

Research output: Contribution to journalArticle

312 Citations (Scopus)

Abstract

Hematopoietic stem cells (HSCs) maintain hematopoiesis by giving rise to all types of blood cells. Recent reports suggest that HSCs also possess the potential to generate nonhematopoietic tissues. To evaluate the underlying mechanisms in the commitment of HSCs into multitissue and multihematopoietic lineages, we performed oligonucleotide array analyses targeting for prospectively purified HSCs, multipotent progenitors (MPPs), common lymphoid progenitors (CLPs), and common myeloid progenitors (CMPs). Here we show that HSCs coexpress multiple nonhematopoietic genes as well as hematopoietic genes; MPPs coexpress myeloid and lymphoid genes; CMPs coexpress myeloerythroid, but not lymphoid genes, whereas CLPs coexpress T-, B-, and natural killer-lymphoid, but not myeloid, genes. Thus, the stepwise decrease in transcriptional accessibility for multilineage-affiliated genes may represent progressive restriction of developmental potentials in early hematopoiesis. These data support the hypothesis that stem cells possess a wide-open chromatin structure to maintain their multipotentiality, which is progressively quenched as they go down a particular pathway of differentiation.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalBlood
Volume101
Issue number2
DOIs
Publication statusPublished - Jan 15 2003

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Hematopoiesis
Stem cells
Hematopoietic Stem Cells
Genes
Tissue
Lymphoid Progenitor Cells
Myeloid Progenitor Cells
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Chromatin
Blood Cells
Blood
Stem Cells
Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis. / Akashi, Koichi; He, Xi; Chen, Jie; Iwasaki, Hiromi; Niu, Chao; Steenhard, Brooke; Zhang, Jiwang; Haug, Jeff; Li, Linheng.

In: Blood, Vol. 101, No. 2, 15.01.2003, p. 383-390.

Research output: Contribution to journalArticle

Akashi, Koichi ; He, Xi ; Chen, Jie ; Iwasaki, Hiromi ; Niu, Chao ; Steenhard, Brooke ; Zhang, Jiwang ; Haug, Jeff ; Li, Linheng. / Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis. In: Blood. 2003 ; Vol. 101, No. 2. pp. 383-390.
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AU - Niu, Chao

AU - Steenhard, Brooke

AU - Zhang, Jiwang

AU - Haug, Jeff

AU - Li, Linheng

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