Transcriptional activators in yeast

Björn Titz, Sindhu Thomas, Seesandra V. Rajagopala, Tomoko Chiba, Takashi Ito, Peter Uetz

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested ∼6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which contained a promoter with a Gal4-binding site. Among the 132 strongest activators 32 are known transcription factors while another 35 have no known function. Although zinc fingers, helix-loop-helix domains and several other domains are highly overrepresented among the activators, only few contain characterized ADs. We also found some striking correlations: the stronger the activation activity, the more acidic, glutamine-rich, proline-rich or asparagine-rich the activators were. About 29% of the activators have been found previously to specifically interact with the transcription machinery, while 10% are known to be components of transcription regulatory complexes. Based on their transcriptional activity, localization and interaction patterns, at least six previously uncharacterized proteins are suggested to be bona fide transcriptional regulators (namely YFL049W, YJR070C, YDR520C, YGL066W/Sgf73, YKR064W and YCR082W/Ahc2).

Original languageEnglish
Pages (from-to)955-967
Number of pages13
JournalNucleic acids research
Volume34
Issue number3
DOIs
Publication statusPublished - Mar 20 2006
Externally publishedYes

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Transcriptional Activation
Helix-Loop-Helix Motifs
Yeasts
Fungal Proteins
Asparagine
Zinc Fingers
Proteome
Glutamine
Reporter Genes
Proline
Transcription Factors
Binding Sites
DNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Titz, B., Thomas, S., Rajagopala, S. V., Chiba, T., Ito, T., & Uetz, P. (2006). Transcriptional activators in yeast. Nucleic acids research, 34(3), 955-967. https://doi.org/10.1093/nar/gkj493

Transcriptional activators in yeast. / Titz, Björn; Thomas, Sindhu; Rajagopala, Seesandra V.; Chiba, Tomoko; Ito, Takashi; Uetz, Peter.

In: Nucleic acids research, Vol. 34, No. 3, 20.03.2006, p. 955-967.

Research output: Contribution to journalArticle

Titz, B, Thomas, S, Rajagopala, SV, Chiba, T, Ito, T & Uetz, P 2006, 'Transcriptional activators in yeast', Nucleic acids research, vol. 34, no. 3, pp. 955-967. https://doi.org/10.1093/nar/gkj493
Titz B, Thomas S, Rajagopala SV, Chiba T, Ito T, Uetz P. Transcriptional activators in yeast. Nucleic acids research. 2006 Mar 20;34(3):955-967. https://doi.org/10.1093/nar/gkj493
Titz, Björn ; Thomas, Sindhu ; Rajagopala, Seesandra V. ; Chiba, Tomoko ; Ito, Takashi ; Uetz, Peter. / Transcriptional activators in yeast. In: Nucleic acids research. 2006 ; Vol. 34, No. 3. pp. 955-967.
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