Transcriptional coactivators CBP and p300 cooperatively enhance HNF-1α-mediated expression of the albumin gene in hepatocytes

Takeaki Dohda, Hidenori Kaneoka, Yujin Inayoshi, Masamichi Kamihira, Katsuhide Miyake, Shinji Iijima

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Transcriptional coactivators, CREB-binding protein (CBP) and p300, exhibit high homology in structure and similar functions. In the present study, we analyzed the function of CBP and p300 proteins as transcriptional coactivators in the expression of albumin in hepatocytes. The expression levels of CBP and p300 were high in fetal hepatocytes, but low in adult ones. Immunoprecipitation assays showed that both CBP and p300 interacted with hepatocyte nuclear factor-1α (HNF-1α) in primary hepatocytes. Furthermore, CBP and p300 were co-precipitated without HNF-1α. Chromatin immunoprecipitation (ChIP) assays revealed that both CBP and p300 are located in the albumin promoter region in hepatocytes. These results suggested that HNF-1α, CBP and p300 were incorporated into a preinitiation complex of RNA polymerase II at the albumin promoter. Luciferase reporter assays showed that CBP and p300 cooperatively triggered HNF-1α-mediated transcription of the albumin promoter. In addition, inhibition of CBP or p300 using small interfering RNAs (siRNAs) resulted in a reduction in albumin expression. These results suggest that both CBP and p300 are required for enhanced expression of albumin.

Original languageEnglish
Pages (from-to)313-319
Number of pages7
JournalJournal of Biochemistry
Volume136
Issue number3
DOIs
Publication statusPublished - Sep 1 2004
Externally publishedYes

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Hepatocyte Nuclear Factor 1
CREB-Binding Protein
Hepatocytes
Albumins
Genes
Gene Expression
Assays
RNA Polymerase II
Chromatin Immunoprecipitation
Transcription
Luciferases
Immunoprecipitation
Genetic Promoter Regions
Small Interfering RNA
Chromatin

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Transcriptional coactivators CBP and p300 cooperatively enhance HNF-1α-mediated expression of the albumin gene in hepatocytes. / Dohda, Takeaki; Kaneoka, Hidenori; Inayoshi, Yujin; Kamihira, Masamichi; Miyake, Katsuhide; Iijima, Shinji.

In: Journal of Biochemistry, Vol. 136, No. 3, 01.09.2004, p. 313-319.

Research output: Contribution to journalArticle

Dohda, Takeaki ; Kaneoka, Hidenori ; Inayoshi, Yujin ; Kamihira, Masamichi ; Miyake, Katsuhide ; Iijima, Shinji. / Transcriptional coactivators CBP and p300 cooperatively enhance HNF-1α-mediated expression of the albumin gene in hepatocytes. In: Journal of Biochemistry. 2004 ; Vol. 136, No. 3. pp. 313-319.
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AU - Miyake, Katsuhide

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