Transcriptional regulation of β-defensin-2 by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells

Junji Mineshiba, Fumio Myokai, Fumi Mineshiba, Kaori Matsuura, Fusanori Nishimura, Shogo Takashiba

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human β-defensin-2 (hBD-2) is an antimicrobial peptide with a broad spectrum of antimicrobial activity against bacteria, yeast and fungi. Here, we analyzed the transcriptional regulation of hBD-2 in cultured human cervical carcinoma (HeLa) cells with or without lipopolysaccharide (LPS). DNA from position -329 to -39 in the hBD-2 promoter region contained the consensus binding sites for transcription factors, one site for nuclear factor for IL-6 expression (NF-IL6) and two sites for nuclear factor-κB (NF-κB). Reporter gene assays for promoter activity revealed that the region had the highest level of responsiveness to LPS. Furthermore, mutations in both of the NF-κB binding sites caused a significant reduction of the responsiveness to LPS, whereas mutation in the NF-IL6 binding site resulted in an elevation of the basal promoter activity. Electrophoretic mobility shift assays demonstrated that LPS induced the binding of HeLa nuclear factors to 60-bp probe containing the two NF-κB binding sites, suggesting that the sites were essential for the binding. Our results suggest that the two NF-κB binding sites contribute to LPS-mediated hBD-2 transcription while the NF-IL6 binding site represses LPS-independent hBD-2 transcription in the HeLa cells.

Original languageEnglish
Pages (from-to)37-44
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume45
Issue number1
DOIs
Publication statusPublished - Jul 1 2005
Externally publishedYes

Fingerprint

Defensins
HeLa Cells
Lipopolysaccharides
Binding Sites
Carcinoma
Interleukin-6
Mutation
Electrophoretic Mobility Shift Assay
Reporter Genes
Genetic Promoter Regions
Fungi
Transcription Factors
Yeasts
Bacteria
Peptides
DNA

All Science Journal Classification (ASJC) codes

  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Transcriptional regulation of β-defensin-2 by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells. / Mineshiba, Junji; Myokai, Fumio; Mineshiba, Fumi; Matsuura, Kaori; Nishimura, Fusanori; Takashiba, Shogo.

In: FEMS Immunology and Medical Microbiology, Vol. 45, No. 1, 01.07.2005, p. 37-44.

Research output: Contribution to journalArticle

Mineshiba, Junji ; Myokai, Fumio ; Mineshiba, Fumi ; Matsuura, Kaori ; Nishimura, Fusanori ; Takashiba, Shogo. / Transcriptional regulation of β-defensin-2 by lipopolysaccharide in cultured human cervical carcinoma (HeLa) cells. In: FEMS Immunology and Medical Microbiology. 2005 ; Vol. 45, No. 1. pp. 37-44.
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