Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust

Shoji Ohga, Daniel J. Royse

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Transcription of laccase and cellulase genes of Lentinula edodes was examined during growth and development under different temperature and moisture levels on a sawdust-based substrate. RNA was extracted from samples of mycelium and fruit bodies at various stages of development and gene expression was determined by competitive RT-PCR. The level of laccase transcripts was maximal during the mycelial growth stage, and then declined rapidly at the fruiting stage. In contrast, the cellulase transcript level peaked at the veil-break stage during fruit body development. Gene expressions for laccase and cellulase were triggered by microclimatic changes, specifically lower temperature and osmotic pressure.

Original languageEnglish
Pages (from-to)111-115
Number of pages5
JournalFEMS microbiology letters
Volume201
Issue number1
DOIs
Publication statusPublished - Jul 10 2001

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Shiitake Mushrooms
Laccase
Cellulase
Fruit
Growth
Genes
Gene Expression
Temperature
Mycelium
Osmotic Pressure
Growth and Development
RNA
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Transcriptional regulation of laccase and cellulase genes during growth and fruiting of Lentinula edodes on supplemented sawdust. / Ohga, Shoji; Royse, Daniel J.

In: FEMS microbiology letters, Vol. 201, No. 1, 10.07.2001, p. 111-115.

Research output: Contribution to journalArticle

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