Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex

Katsuhide Miyake, Mikio Takahashi, Takeaki Dohda, Hidenori Kaneoka, Yoshitaka Sato, Yujin Inayoshi, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticle

Abstract

α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter-proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalCytotechnology
Volume49
Issue number2-3
DOIs
Publication statusPublished - Oct 1 2005
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Biotechnology
  • Clinical Biochemistry

Cite this

Miyake, K., Takahashi, M., Dohda, T., Kaneoka, H., Sato, Y., Inayoshi, Y., ... Iijima, S. (2005). Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex. Cytotechnology, 49(2-3), 143-151. https://doi.org/10.1007/s10616-006-6702-2