Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex

Katsuhide Miyake, Mikio Takahashi, Takeaki Dohda, Hidenori Kaneoka, Yoshitaka Sato, Yujin Inayoshi, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticle

Abstract

α-Fetoprotein (AFP) is a fetal serum protein abundant in fetal liver whose expression is downregulated in adult liver. The promoter-proximal region of the AFP gene contains two binding sites for CCAAT/enhancer-binding protein α (C/EBPα), two for hepatocyte nuclear factor-1α (HNF-1α) and one for nuclear factor-1 (NF-1). Luciferase reporter assays showed that a combination of C/EBPα, HNF-1α, NF-1 and coactivator p300 gave the maximal activity but the BRG1 and BRM, ATPase subunit of the chromatin remodeling complex SWI/SNF, repressed the transactivation of the AFP gene by these transcription factors. Deletion analyses of C/EBPα binding sites suggested that C/EBPα recruited SWI/SNF complex and caused the repression. This repression may also play important roles in the downregulation of the AFP gene in adult hepatocytes.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalCytotechnology
Volume49
Issue number2-3
DOIs
Publication statusPublished - Oct 1 2005
Externally publishedYes

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CCAAT-Enhancer-Binding Proteins
Fetal Proteins
Chromatin Assembly and Disassembly
Chromatin
Hepatocyte Nuclear Factor 1
Genes
NFI Transcription Factors
Binding sites
Liver
Down-Regulation
Binding Sites
Transcription factors
Luciferases
Genetic Promoter Regions
Protein Binding
Transcriptional Activation
Adenosine Triphosphatases
Blood Proteins
Hepatocytes
Assays

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Biotechnology
  • Clinical Biochemistry

Cite this

Miyake, K., Takahashi, M., Dohda, T., Kaneoka, H., Sato, Y., Inayoshi, Y., ... Iijima, S. (2005). Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex. Cytotechnology, 49(2-3), 143-151. https://doi.org/10.1007/s10616-006-6702-2

Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex. / Miyake, Katsuhide; Takahashi, Mikio; Dohda, Takeaki; Kaneoka, Hidenori; Sato, Yoshitaka; Inayoshi, Yujin; Kamihira, Masamichi; Iijima, Shinji.

In: Cytotechnology, Vol. 49, No. 2-3, 01.10.2005, p. 143-151.

Research output: Contribution to journalArticle

Miyake, K, Takahashi, M, Dohda, T, Kaneoka, H, Sato, Y, Inayoshi, Y, Kamihira, M & Iijima, S 2005, 'Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex', Cytotechnology, vol. 49, no. 2-3, pp. 143-151. https://doi.org/10.1007/s10616-006-6702-2
Miyake K, Takahashi M, Dohda T, Kaneoka H, Sato Y, Inayoshi Y et al. Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex. Cytotechnology. 2005 Oct 1;49(2-3):143-151. https://doi.org/10.1007/s10616-006-6702-2
Miyake, Katsuhide ; Takahashi, Mikio ; Dohda, Takeaki ; Kaneoka, Hidenori ; Sato, Yoshitaka ; Inayoshi, Yujin ; Kamihira, Masamichi ; Iijima, Shinji. / Transcriptional regulation of the α-fetoprotein gene by SWI/SNF chromatin remodeling complex. In: Cytotechnology. 2005 ; Vol. 49, No. 2-3. pp. 143-151.
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