Transcriptome complexity in a genome-reduced bacterium

Marc Güell, Vera Van Noort, Eva Yus, Wei Hua Chen, Justine Leigh-Bell, Konstantinos Michalodimitrakis, Takuji Yamada, Manimozhiyan Arumugam, Tobias Doerks, Sebastian Kühner, Michaela Rode, Mikita Suyama, Sabine Schmidt, Anne Claude Gavin, Peer Bork, Luis Serrano

Research output: Contribution to journalArticle

303 Citations (Scopus)

Abstract

To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms. Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

Original languageEnglish
Pages (from-to)1268-1271
Number of pages4
JournalScience
Volume326
Issue number5957
DOIs
Publication statusPublished - Nov 27 2009
Externally publishedYes

Fingerprint

Transcriptome
Genome
Operon
Bacteria
Mycoplasma pneumoniae
Regulator Genes
Eukaryota
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Güell, M., Van Noort, V., Yus, E., Chen, W. H., Leigh-Bell, J., Michalodimitrakis, K., ... Serrano, L. (2009). Transcriptome complexity in a genome-reduced bacterium. Science, 326(5957), 1268-1271. https://doi.org/10.1126/science.1176951

Transcriptome complexity in a genome-reduced bacterium. / Güell, Marc; Van Noort, Vera; Yus, Eva; Chen, Wei Hua; Leigh-Bell, Justine; Michalodimitrakis, Konstantinos; Yamada, Takuji; Arumugam, Manimozhiyan; Doerks, Tobias; Kühner, Sebastian; Rode, Michaela; Suyama, Mikita; Schmidt, Sabine; Gavin, Anne Claude; Bork, Peer; Serrano, Luis.

In: Science, Vol. 326, No. 5957, 27.11.2009, p. 1268-1271.

Research output: Contribution to journalArticle

Güell, M, Van Noort, V, Yus, E, Chen, WH, Leigh-Bell, J, Michalodimitrakis, K, Yamada, T, Arumugam, M, Doerks, T, Kühner, S, Rode, M, Suyama, M, Schmidt, S, Gavin, AC, Bork, P & Serrano, L 2009, 'Transcriptome complexity in a genome-reduced bacterium', Science, vol. 326, no. 5957, pp. 1268-1271. https://doi.org/10.1126/science.1176951
Güell M, Van Noort V, Yus E, Chen WH, Leigh-Bell J, Michalodimitrakis K et al. Transcriptome complexity in a genome-reduced bacterium. Science. 2009 Nov 27;326(5957):1268-1271. https://doi.org/10.1126/science.1176951
Güell, Marc ; Van Noort, Vera ; Yus, Eva ; Chen, Wei Hua ; Leigh-Bell, Justine ; Michalodimitrakis, Konstantinos ; Yamada, Takuji ; Arumugam, Manimozhiyan ; Doerks, Tobias ; Kühner, Sebastian ; Rode, Michaela ; Suyama, Mikita ; Schmidt, Sabine ; Gavin, Anne Claude ; Bork, Peer ; Serrano, Luis. / Transcriptome complexity in a genome-reduced bacterium. In: Science. 2009 ; Vol. 326, No. 5957. pp. 1268-1271.
@article{d11060b772f149698997b4beb8e17558,
title = "Transcriptome complexity in a genome-reduced bacterium",
abstract = "To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms. Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.",
author = "Marc G{\"u}ell and {Van Noort}, Vera and Eva Yus and Chen, {Wei Hua} and Justine Leigh-Bell and Konstantinos Michalodimitrakis and Takuji Yamada and Manimozhiyan Arumugam and Tobias Doerks and Sebastian K{\"u}hner and Michaela Rode and Mikita Suyama and Sabine Schmidt and Gavin, {Anne Claude} and Peer Bork and Luis Serrano",
year = "2009",
month = "11",
day = "27",
doi = "10.1126/science.1176951",
language = "English",
volume = "326",
pages = "1268--1271",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5957",

}

TY - JOUR

T1 - Transcriptome complexity in a genome-reduced bacterium

AU - Güell, Marc

AU - Van Noort, Vera

AU - Yus, Eva

AU - Chen, Wei Hua

AU - Leigh-Bell, Justine

AU - Michalodimitrakis, Konstantinos

AU - Yamada, Takuji

AU - Arumugam, Manimozhiyan

AU - Doerks, Tobias

AU - Kühner, Sebastian

AU - Rode, Michaela

AU - Suyama, Mikita

AU - Schmidt, Sabine

AU - Gavin, Anne Claude

AU - Bork, Peer

AU - Serrano, Luis

PY - 2009/11/27

Y1 - 2009/11/27

N2 - To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms. Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

AB - To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms. Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

UR - http://www.scopus.com/inward/record.url?scp=70849119931&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70849119931&partnerID=8YFLogxK

U2 - 10.1126/science.1176951

DO - 10.1126/science.1176951

M3 - Article

C2 - 19965477

AN - SCOPUS:70849119931

VL - 326

SP - 1268

EP - 1271

JO - Science

JF - Science

SN - 0036-8075

IS - 5957

ER -