Transcriptome complexity in a genome-reduced bacterium

Marc Güell, Vera Van Noort, Eva Yus, Wei Hua Chen, Justine Leigh-Bell, Konstantinos Michalodimitrakis, Takuji Yamada, Manimozhiyan Arumugam, Tobias Doerks, Sebastian Kühner, Michaela Rode, Mikita Suyama, Sabine Schmidt, Anne Claude Gavin, Peer Bork, Luis Serrano

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304 Citations (Scopus)

Abstract

To study basic principles of transcriptome organization in bacteria, we analyzed one of the smallest self-replicating organisms. Mycoplasma pneumoniae. We combined strand-specific tiling arrays, complemented by transcriptome sequencing, with more than 252 spotted arrays. We detected 117 previously undescribed, mostly noncoding transcripts, 89 of them in antisense configuration to known genes. We identified 341 operons, of which 139 are polycistronic; almost half of the latter show decaying expression in a staircase-like manner. Under various conditions, operons could be divided into 447 smaller transcriptional units, resulting in many alternative transcripts. Frequent antisense transcripts, alternative transcripts, and multiple regulators per gene imply a highly dynamic transcriptome, more similar to that of eukaryotes than previously thought.

Original languageEnglish
Pages (from-to)1268-1271
Number of pages4
JournalScience
Volume326
Issue number5957
DOIs
Publication statusPublished - Nov 27 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

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    Güell, M., Van Noort, V., Yus, E., Chen, W. H., Leigh-Bell, J., Michalodimitrakis, K., Yamada, T., Arumugam, M., Doerks, T., Kühner, S., Rode, M., Suyama, M., Schmidt, S., Gavin, A. C., Bork, P., & Serrano, L. (2009). Transcriptome complexity in a genome-reduced bacterium. Science, 326(5957), 1268-1271. https://doi.org/10.1126/science.1176951