Transcriptome dynamics of rooting zone and aboveground parts of cuttings during adventitious root formation in Cryptomeria japonica D. Don

Yuki Fukuda, Tomonori Hirao, Kentaro Mishima, Mineko Ohira, Yuichiro Hiraoka, Makoto Takahashi, Atsushi Watanabe

Research output: Contribution to journalArticle

Abstract

Background: Adventitious root formation is an essential physiological process for successful propagation of cuttings in various plant species. Because coniferous species are highly heterozygous, propagation of cuttings is of great practical use in breeding. Although various factors influence adventitious root formation, little is known of the associated regulatory mechanisms. Whereas adventitious roots generally form from the base of cuttings, this process is accompanied by physiological changes in leaves, which supply assimilates and metabolites. Herein, we present microarray analyses of transcriptome dynamics during adventitious root formation in whole cuttings in the coniferous species, Cryptomeria japonica. Results: Temporal patterns of gene expression were determined in the base, the middle, and needles of cuttings at eight time points during adventitious root formation. Global gene expression at the base had diverged from that in the middle by 3-h post-insertion, and changed little in the subsequent 3-days post-insertion, and global gene expression in needles altered characteristically at 3- and 6-weeks post-insertion. In Gene Ontology enrichment analysis of major gene clusters based on hierarchical clustering, the expression profiles of genes related to carbohydrates, plant hormones, and other categories indicated multiple biological changes that were involved in adventitious root formation. Conclusions: The present comprehensive transcriptome analyses indicate major transcriptional turning and contribute to the understanding of the biological processes and molecular factors that influence adventitious root formation in C. japonica.

Original languageEnglish
Article number201
JournalBMC plant biology
Volume18
Issue number1
DOIs
Publication statusPublished - Sep 19 2018

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Cryptomeria japonica
adventitious roots
transcriptome
aerial parts
rooting
gene expression
plant cuttings
major genes
multigene family
plant hormones
genes
metabolites
carbohydrates
breeding
leaves

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Transcriptome dynamics of rooting zone and aboveground parts of cuttings during adventitious root formation in Cryptomeria japonica D. Don. / Fukuda, Yuki; Hirao, Tomonori; Mishima, Kentaro; Ohira, Mineko; Hiraoka, Yuichiro; Takahashi, Makoto; Watanabe, Atsushi.

In: BMC plant biology, Vol. 18, No. 1, 201, 19.09.2018.

Research output: Contribution to journalArticle

Fukuda, Yuki ; Hirao, Tomonori ; Mishima, Kentaro ; Ohira, Mineko ; Hiraoka, Yuichiro ; Takahashi, Makoto ; Watanabe, Atsushi. / Transcriptome dynamics of rooting zone and aboveground parts of cuttings during adventitious root formation in Cryptomeria japonica D. Don. In: BMC plant biology. 2018 ; Vol. 18, No. 1.
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