Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis

Eiji Nagayasu, Yoshitoshi Ogura, Takehiko Itoh, Ayako Yoshida, Gunimala Chakraborty, Tetsuya Hayashi, Haruhiko Maruyama

Research output: Contribution to journalArticle

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Abstract

Strongyloides venezuelensis is one of some 50 species of genus Strongyloides, obligate gastrointestinal parasites of vertebrates, responsible for strongyloidiasis in humans and other domestic/companion animals. Although S. venezuelensis has been widely used as a model species for studying human/animal strongyloidiasis, the sequence information for this species has been quite limited. To create a more comprehensive catalogue of expressed genes for identification of genes potentially involved in animal parasitism, we conducted a de novo sequencing analysis of the transcriptomes from four developmental stages of S. venezuelensis, using a Roche 454 GS FLX Titanium pyrosequencing platform. A total of 14,573 contigs were produced after de novo assemblies of over 2. million sequencing reads and formed a dataset "Vene454". BLAST homology search of Vene454 against proteome and transcriptome data from other animal-parasitic and non-animal-parasitic nematode species revealed several interesting genes, which may be potentially related to animal parasitism, including nicotinamide phosphoribosyltransferase and ferrochelatase. The Vene454 dataset analysis also enabled us to identify transcripts that are specifically enriched in each developmental stage. This work represents the first large-scale transcriptome analysis of S. venezuelensis and the first study to examine the transcriptome of the lung L3 developmental stage of any Strongyloides species. The results not only will serve as valuable resources for future functional genomics analyses to understand the molecular aspects of animal parasitism, but also will provide essential information for ongoing whole genome sequencing efforts in this species.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalParasitology International
Volume62
Issue number1
DOIs
Publication statusPublished - Feb 1 2013
Externally publishedYes

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Strongyloides
Strongyloidiasis
Gene Expression Profiling
Transcriptome
Ferrochelatase
Nicotinamide Phosphoribosyltransferase
Genes
Pets
Domestic Animals
Proteome
Genomics
Titanium
Vertebrates
Parasites
Genome
Lung

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Cite this

Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis. / Nagayasu, Eiji; Ogura, Yoshitoshi; Itoh, Takehiko; Yoshida, Ayako; Chakraborty, Gunimala; Hayashi, Tetsuya; Maruyama, Haruhiko.

In: Parasitology International, Vol. 62, No. 1, 01.02.2013, p. 57-65.

Research output: Contribution to journalArticle

Nagayasu, Eiji ; Ogura, Yoshitoshi ; Itoh, Takehiko ; Yoshida, Ayako ; Chakraborty, Gunimala ; Hayashi, Tetsuya ; Maruyama, Haruhiko. / Transcriptomic analysis of four developmental stages of Strongyloides venezuelensis. In: Parasitology International. 2013 ; Vol. 62, No. 1. pp. 57-65.
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