Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast

Tohru Ichimura, Hiroyuki Kubota, Takeshi Goma, Noboru Mizushima, Yoshinori Ohsumi, Maki Iwago, Kazue Kakiuchi, Hossain Uddin Shekhar, Takashi Shinkawa, Masato Taoka, Takashi Ito, Toshiaki Isobe

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33 Citations (Scopus)

Abstract

BMH1 and BMH2 encode Saccharomyces cerevisiae 14-3-3 homologues whose exact functions have remained unclear. The present work compares the transcriptomic and proteomic profiles of the wild type and a BMH1/2-deficient S. cerevisiae mutant (bmhΔ) using DNA microarrays and two-dimensional polyacrylamide gel electrophoresis. It is reported here that, although the global patterns of gene and protein expression are very similar between the two types of yeast cells, a subset of genes and proteins (a total of 220 genes) is significantly induced or reduced in the absence of Bmh1/2p. These genes include approximately 60 elements that could be linked to the reported phenotypes of the bmhΔ mutant (e.g., accumulation of glycogen and hypersensitivity to environmental stress) and/or could be the potential downstream targets of interacting partners of Bmh1/2p such as Msn2p and Rtg3p. Importantly, >30% of the identified genes (71 genes) were found to be associated with carbon (C) and nitrogen (N) metabolism and transport, thereby suggesting that Bmh1/2p may play a major role in the regulation of C/N-responsive cellular processes. This study presents the first comprehensive overview of the genes and proteins that are affected by the depletion of Bmh1/2p and extends the scope of knowledge of the regulatory roles of Bmh1/2p in S. cerevisiae.

Original languageEnglish
Pages (from-to)6149-6158
Number of pages10
JournalBiochemistry
Volume43
Issue number20
DOIs
Publication statusPublished - May 25 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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    Ichimura, T., Kubota, H., Goma, T., Mizushima, N., Ohsumi, Y., Iwago, M., Kakiuchi, K., Shekhar, H. U., Shinkawa, T., Taoka, M., Ito, T., & Isobe, T. (2004). Transcriptomic and proteomic analysis of a 14-3-3 gene-deficient yeast. Biochemistry, 43(20), 6149-6158. https://doi.org/10.1021/bi035421i