Transdermal delivery of the anti-rheumatic agent methotrexate using a solid-in-oil nanocarrier

Research output: Contribution to journalArticle

23 Citations (Scopus)


Transdermal delivery of methotrexate (MTX) was investigated by using the solid-in-oil (S/O) technique. Because MTX was coated with nonionic surfactant molecules, the resulting complex was easy to dissolve in various organic solvents and provided a transparent solution in isopropyl myristate (IPM). The stability of MTX-surfactant complexes are enhanced by the addition of a basic amino acid such as l-Arginine (l-Arg) or l-Lysine (l-Lys). The average size of the dispersed complex of MTX and amino acid was reduced to below 100 nm and gave a uniform distribution. A transdermal delivery experiment was conducted using the S/O nanocarrier, and the permeation behavior of MTX through Yucatan micropig (YMP) skin was evaluated with a Franz diffusion cell. The permeation efficiency for the S/O nanocarrier (not urea addition) was two- to threefold increased compared to that of the control aqueous solution because the oil-based nanocarrier is effective for penetrating the stratum corneum. Furthermore, addition of urea has dramatically improved the release property of MTX from the S/O nanocarrier, and the S/O nanocarrier containing urea showed an optimal permeation efficiency of approximately 8.8-fold increased compared to that of the control aqueous solution after 24 h (p < 0.01).

Original languageEnglish
Pages (from-to)158-163
Number of pages6
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Issue number1
Publication statusPublished - Sep 1 2012


All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Pharmaceutical Science

Cite this